Differential protein expression in pancreatic islets after treatment with an imidazoline compound

  • T. Jägerbrink
  • H. Lexander
  • C. Palmberg
  • J. Shafqat
  • V. Sharoyko
  • P.-O. Berggren
  • S. Efendic
  • S. Zaitsev
  • H. Jörnvall
Research Article

DOI: 10.1007/s00018-007-7136-5

Cite this article as:
Jägerbrink, T., Lexander, H., Palmberg, C. et al. Cell. Mol. Life Sci. (2007) 64: 1310. doi:10.1007/s00018-007-7136-5

Abstract.

The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1).

Keywords.

2-D gel electrophoresis pancreatic islets proteomics type 2 diabetes imidazolines BL11282 

Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • T. Jägerbrink
    • 1
  • H. Lexander
    • 2
  • C. Palmberg
    • 1
  • J. Shafqat
    • 1
  • V. Sharoyko
    • 3
  • P.-O. Berggren
    • 3
  • S. Efendic
    • 3
  • S. Zaitsev
    • 3
    • 4
  • H. Jörnvall
    • 1
  1. 1.Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSweden
  2. 2.Department of Oncology and PathologyKarolinska InstitutetStockholmSweden
  3. 3.The Rolf Luft Research Center for Diabetes and EndocrinologyKarolinska InstitutetStockholmSweden
  4. 4.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

Personalised recommendations