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Differential protein expression in pancreatic islets after treatment with an imidazoline compound

  • T. Jägerbrink
  • H. Lexander
  • C. Palmberg
  • J. Shafqat
  • V. Sharoyko
  • P.-O. Berggren
  • S. Efendic
  • S. Zaitsev
  • H. JörnvallEmail author
Research Article

Abstract.

The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1).

Keywords.

2-D gel electrophoresis pancreatic islets proteomics type 2 diabetes imidazolines BL11282 

Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • T. Jägerbrink
    • 1
  • H. Lexander
    • 2
  • C. Palmberg
    • 1
  • J. Shafqat
    • 1
  • V. Sharoyko
    • 3
  • P.-O. Berggren
    • 3
  • S. Efendic
    • 3
  • S. Zaitsev
    • 3
    • 4
  • H. Jörnvall
    • 1
    Email author
  1. 1.Department of Medical Biochemistry and BiophysicsKarolinska InstitutetStockholmSweden
  2. 2.Department of Oncology and PathologyKarolinska InstitutetStockholmSweden
  3. 3.The Rolf Luft Research Center for Diabetes and EndocrinologyKarolinska InstitutetStockholmSweden
  4. 4.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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