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Cellular and Molecular Life Sciences CMLS

, Volume 63, Issue 18, pp 2175–2182 | Cite as

Cholinesterases are down-expressed in human colorectal carcinoma

  • M. F. Montenegro
  • F. Ruiz-Espejo
  • F. J. Campoy
  • E. Muñoz-Delgado
  • M. Páez de la Cadena
  • F. J. Rodríguez-Berrocal
  • C. J. Vidal
Research Article

Abstract.

The aberrations of cholinesterase (ChE) genes and the variation of ChE activity in cancerous tissues prompted us to investigate the expression of ChEs in colorectal carcinoma. The study of 55 paired specimens of healthy (HG) and cancerous gut (CG) showed that acetylcholinesterase (AChE) activity fell by 32% and butyrylcholinesterase (BuChE) activity by 58% in CG. Abundant AChE-H, fewer AChE-T, and even fewer AChE-R and BuChE mRNAs were observed in HG, and their content was greatly diminished in CG. The high level of the AChE-H mRNA explains the abundance of AChE-H subunits in HG, which as glycosylphosphatidylinositol (GPI)-anchored amphiphilic AChE dimers (G 2 A ) and monomers (G 1 A ) account for 69% of AChE activity. The identification of AChE-T and BuChE mRNAs justifies the occurrence in gut of A12, G 4 H and PRiMA-containing G 4 A AChE forms, besides G 4 H , G 4 A and G 1 H BuChE. The down-regulation of ChEs might contribute to gut carcinogenesis by increasing acetylcholine availability and overstimulating muscarinic receptors.

Keywords.

Cancer cholinesterases glycosylphosphatidylinositol gut real-time PCR 

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Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  • M. F. Montenegro
    • 1
    • 2
  • F. Ruiz-Espejo
    • 1
    • 3
  • F. J. Campoy
    • 1
  • E. Muñoz-Delgado
    • 1
  • M. Páez de la Cadena
    • 2
  • F. J. Rodríguez-Berrocal
    • 2
  • C. J. Vidal
    • 1
  1. 1.Departamento de Bioquímica y Biología Molecular-A, Edificio de VeterinariaUniversidad de MurciaMurciaSpain
  2. 2.Departamento de Bioquímica, Genética e Inmunología, Facultad de BiologíaUniversidad de VigoVigoSpain
  3. 3.Servicio de Análisis ClínicosHospital Virgen de la ArrixacaMurciaSpain

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