Cellular and Molecular Life Sciences CMLS

, Volume 62, Issue 18, pp 2132–2139

Identification of factor XIII-A as a marker of alternative macrophage activation

Research Article

Abstract.

Factor XIII subunit A of blood coagulation (FXIII-A) is known to be synthesized but not secreted by the monocyte/macrophage cell line. On the basis of its intracellular localization and substrate profile, FXIII-A is thought to be involved in certain intracellular processes. Our present study was designed to monitor the changes in FXIII-A gene expression and protein production in long-term culture of human monocytes during their differentiation into macrophages in the presence of activating agents (interleukin-4, interferon-γ, Mycobacterium bovis BCG) inducing classical and alternative activation pathways. By using quantitative RT-PCR and fluorescent image analysis at the single-cell level we demonstrated that the expression of FXIII-A both at the mRNA as well as at the protein level is inversely regulated during the two activation programmes. Here we conclude that FXIII-A expression is an intracellular marker for alternatively activated macrophages, while its absence in monocyte-derived macrophages indicates their classically activated state.

Key words.

Factor XIII-A macrophage gene expression alternative activation classical activation 

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Copyright information

© Birkhäuser Verlag, Basel 2005

Authors and Affiliations

  • D. Törőcsik
    • 1
  • H. Bárdos
    • 2
  • L. Nagy
    • 1
  • R. Ádány
    • 2
  1. 1.Department of Biochemistry and Molecular Biology, Faculty of Medicine, Medical and Health Science CentreUniversity of DebrecenDebrecenHungary
  2. 2.Department of Preventive Medicine, School of Public Health, Medical and Health Science CentreUniversity of DebrecenDebrecenHungary

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