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Cellular and Molecular Life Sciences CMLS

, Volume 62, Issue 15, pp 1755–1762 | Cite as

Human peripheral blood monuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro

  • R. Teufel
  • J.-P. Carralot
  • B. Scheel
  • J. Probst
  • S. Walter
  • G. Jung
  • I. Hoerr
  • H.-G. Rammensee
  • S. PascoloEmail author
Research Article

Abstract.

The efficiency of test vaccines needs to be evaluated by quantification of the triggered cellular immune response. Usually, for these assays, autologous target cells expressing the vaccine antigen are required. In the context of messenger RNA (mRNA)-based vaccinations, the target cells used for the read-out are mRNA-transfected monocyte-derived dendritic cells (Mo-DCs). Their production typically requires samples of 100 ml blood from the patients, and limits the number of assays that can be performed. We show here that fresh peripheral blood mononuclear cells (PBMCs) can be transfected with mRNA by electroporation. Such cells are as efficient as mRNA-transfected Mo-DCs for their ability to activate memory T cells in vitro. Thus, mRNA-transfected PBMCs are a convenient replacement of mRNA-transfected Mo-DCs for the in vitro monitoring of natural or vaccine-induced immune responses.

Key words.

mRNA B lymphocyte dendritic cell Monocyte T lymphocyte MHC tetramer 

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Copyright information

© Birkhäuser Verlag, Basel 2005

Authors and Affiliations

  • R. Teufel
    • 1
  • J.-P. Carralot
    • 1
    • 2
  • B. Scheel
    • 1
  • J. Probst
    • 2
  • S. Walter
    • 2
  • G. Jung
    • 3
  • I. Hoerr
    • 1
  • H.-G. Rammensee
    • 1
  • S. Pascolo
    • 1
    • 2
    Email author
  1. 1.CureVac GmbHTübingenGermany
  2. 2.Department of ImmunologyInstitute for Cell BiologyTübingenGermany
  3. 3.Institute for Organic ChemistryTübingenGermany

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