Cellular and Molecular Life Sciences CMLS

, Volume 62, Issue 2, pp 239–250 | Cite as

Oestrogen-modulated gene expression in the human endometrium

  • C. Punyadeera
  • H. Dassen
  • J. Klomp
  • G. Dunselman
  • R. Kamps
  • F. Dijcks
  • A. Ederveen
  • A. de Goeij
  • P. Groothuis
Research Article

Abstract.

To identify key regulatory mechanisms in the growth and development of the human endometrium, microarray analysis was performed on uncultured human endometrium collected during menstruation (M) and the late-proliferative (LATE-P)-phase of the menstrual cycle, as well as after 24 h incubation in the presence of oestradiol (17β-E2). We demonstrate the expression of novel gene transcripts in the human endometrium. i.e. mucin-9, novel oestrogen-responsive gene transcripts, i.e. gelsolin and flotillin-1, and genes known to be expressed in human endometrium but not yet shown to be oestrogen responsive, i.e. connexin-37 and TFF1/pS2. Genes reported to be expressed during the implantation window and implicated in progesterone action, i.e. secretoglobin family 2A, member 2 (mammaglobin) and homeobox-containing proteins, were up-regulated in uncultured LATE-P-phase endometrium compared to M-phase endometrium. Some gene transcripts are regulated directly by 17β-E2 alone, others are influenced by the in vivo environment as well. These observations emphasise that the regulation of endometrium maturation by oestrogen entails more then just stimulation of cell proliferation.

Key words.

Steroid hormones cell differentiation extracellular matrix remodelling angiogenesis physiology 

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Copyright information

© Birkhäuser Verlag, Basel 2005

Authors and Affiliations

  • C. Punyadeera
    • 1
    • 2
  • H. Dassen
    • 2
  • J. Klomp
    • 3
  • G. Dunselman
    • 1
    • 4
  • R. Kamps
    • 1
    • 2
  • F. Dijcks
    • 5
  • A. Ederveen
    • 5
  • A. de Goeij
    • 1
    • 2
  • P. Groothuis
    • 1
    • 4
  1. 1.Research Institute GROWAZ MaastrichtThe Netherlands
  2. 2.Departments of Pathology MD MaastrichtThe Netherlands
  3. 3.Department of PharmacogenomicsN.V. OrganonOssThe Netherlands
  4. 4.Department of Obstetrics and GynaecologyUniversity HospitalMaastrichtThe Netherlands
  5. 5.Department of PharmacologyN.V. OrganonOssThe Netherlands

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