Cellular and Molecular Life Sciences CMLS

, Volume 60, Issue 8, pp 1529–1546

Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint of large-scale production and purification

Multi-author Review

DOI: 10.1007/s00018-003-3168-7

Cite this article as:
Sarramegna, V., Talmont, F., Demange, P. et al. CMLS, Cell. Mol. Life Sci. (2003) 60: 1529. doi:10.1007/s00018-003-3168-7


G-protein-coupled receptors (GPCRs) are of prime importance for cell signal transduction mechanisms and are the target of many current and potential drugs. However, structural data on these membrane proteins is still scarce because of their low natural abundance and the low efficiency of most of the expression systems currently available. This review presents the most important expression systems currently employed for heterologous expression of GPCRs; Escherichia coli, yeast, insect cells and mammalian cells. After briefly recalling the specificity, advantages and limitations of each system, particular emphasis is put on the quantitative comparison of these expression systems in terms of overall expression yield, and on the influence of various factors (primary sequence, origin, cell type, N- and C-terminal tags) on the results.

GPCRs heterologous expression membrane proteins receptor Pichia pastoris NMR 

Copyright information

© Birkhäuser-Verlag Basel 2003

Authors and Affiliations

  • V. Sarramegna
    • 1
  • F. Talmont
    • 1
  • P. Demange
    • 1
  • A. Milon
    • 1
  1. 1.UMR 5089Institut de Pharmacologie et de Biologie StructuraleToulousecedex 4

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