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Cellular and Molecular Life Sciences CMLS

, Volume 60, Issue 8, pp 1760–1763 | Cite as

Green tea epigallocatechin-3-gallate is an inhibitor of mammalian histidine decarboxylase

  • C. Rodríguez-Caso
  • D. Rodríguez-Agudo
  • F. Sánchez-Jiménez
  • M. A. Medina
Research Article

Abstract

(–)-Epigallocatechin-3-gallate, an antiproliferative and antiangiogenic component of green tea, has been reported to inhibit dopa decarboxylase. In this report, we show that this compound also inhibits histidine decarboxylase, the enzymic activity responsible for histamine biosynthesis. This inhibition was proved by a double approach, activity measurements and UV-Vis spectra of enzyme-bound pyridoxal-5′-phosphate. At 0.1mM (–)-epigallocatechin-3-gallate, histidine decarboxylase activity was inhibited by more than 60% and the typical spectrum of the internal aldimine form shifted to a stable major maximum at 345nm, suggesting that the compound causes a stable change in the structure of the holoenzyme. Since histamine release is one of the primary events in many inflammatory responses, a new potential application of (–)-epigallocatechin-3-gallate in prevention or treatment of inflammatory processes is suggested by these data.

Histidine decarboxylase epigallocatechin-3-gallate pyridoxal-5′-phosphate histamine green tea 

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Copyright information

© Birkhäuser-Verlag Basel 2003

Authors and Affiliations

  • C. Rodríguez-Caso
    • 1
  • D. Rodríguez-Agudo
    • 1
  • F. Sánchez-Jiménez
    • 1
  • M. A. Medina
    • 1
  1. 1.Department of Molecular Biology and BiochemistryFaculty of Sciences, University of MálagaMálaga

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