Antisense-mediated redirection of mRNA splicing
- Cite this article as:
- Vacek, M., Sazani, P. & Kole, R. CMLS, Cell. Mol. Life Sci. (2003) 60: 825. doi:10.1007/s00018-003-3042-7
Antisense technology has been used to study basic biological processes, and to block these processes when they deleteriously lead to human disease. A separate, equally important application of antisense technology is to upregulate the gene expression lost in the diseased state by shifting alternative splicing of pre-messenger RNA. This strategy has commonly relied upon the use of antisense oligonucleotides; however, another approach is to use a plasmid construct to generate antisense RNA inside the cell. Antisense therapeutics based on expression vectors and viral vectors offers a gene therapy approach, whereas those based on oligonucleotides offers a more drug like approach.