Cellular and Molecular Life Sciences CMLS

, Volume 60, Issue 5, pp 825–833

Antisense-mediated redirection of mRNA splicing

  • M. Vacek
  • P. Sazani
  • R. Kole
Multi-author Review

DOI: 10.1007/s00018-003-3042-7

Cite this article as:
Vacek, M., Sazani, P. & Kole, R. CMLS, Cell. Mol. Life Sci. (2003) 60: 825. doi:10.1007/s00018-003-3042-7

Abstract:

Antisense technology has been used to study basic biological processes, and to block these processes when they deleteriously lead to human disease. A separate, equally important application of antisense technology is to upregulate the gene expression lost in the diseased state by shifting alternative splicing of pre-messenger RNA. This strategy has commonly relied upon the use of antisense oligonucleotides; however, another approach is to use a plasmid construct to generate antisense RNA inside the cell. Antisense therapeutics based on expression vectors and viral vectors offers a gene therapy approach, whereas those based on oligonucleotides offers a more drug like approach.

Key words: Antisense; splicing; oligonucleotides; snRNAs; gene therapy. 

Copyright information

© Birkhäuser Verlag, 2003

Authors and Affiliations

  • M. Vacek
    • 1
  • P. Sazani
    • 2
  • R. Kole
    • 1
  1. 1.Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina (USA)US
  2. 2.Department of Pharmacology, University of North Carolina, Chapel Hill, North Carolina (USA)US
  3. 3.Lineberger Comprehensive Cancer Center, CB # 7295, University of North Carolina, Chapel Hill, North Carolina 27599-7295 (USA), Fax: + 1 919 966 3015, e-mail: kole@med.unc.eduUS

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