Inflammation Research

, Volume 50, Issue 1, pp 44–49 | Cite as

Dithranol upregulates IL-10 receptors on the cultured human keratinocyte cell line HaCaT

  • Á Farkas
  • L. Kemény
  • B.J. Szöny
  • Zs. Bata-Csörgö
  • A. Pivarcsi
  • M. Kiss
  • M. Széll
  • A. Koreck
  • A. Dobozy

Abstract:

Objective: Dithranol is highly effective in the treatment of psoriasis, however its mode of action is still not well known. Since interleukin-8 and interleukin-10 are involved in the pathogenesis of psoriasis, the aim of our study was to investigate the effect of dithranol on interleukin-8, interleukin-10 mRNA production and interleukin-10 receptor expression of the HaCaT keratinocyte cell line which is commonly used in experiments examining the effects of therapeutic drugs on keratinocytes.¶Materials and Methods: Cultured HaCaT cells were treated with 0.1-0.5 μg/ml dithranol for 30 minutes. After 2 and 4 h total cellular RNA isolated from HaCaT cells was reverse transcribed (RT) to cDNA which was subjected to polymerase chain reaction (PCR) with specific primer pairs for interleukin-8, interleukin-10 and interleukin-10 receptor. For immunohistochemistry cultured HaCaT cells were stained with a monoclonal antibody against the human interleukin-10 receptor.¶Results: Our results showed that dithranol treatment did not change the highly elevated level of interleukin-8 mRNA of HaCaT cells. Interleukin-10 mRNA signal with RT-PCR could not be detected in HaCaT cells. Depending on the concentration dithranol increased the mRNA production of interleukin-10 receptors in HaCaT cells. This dithranol induced dose dependent upregulation of IL-10 receptors in HaCaT cells was also observed on the protein level using immunohistochemistry.¶Conclusions: Since the interleukin-10 receptor expression of keratinocytes in psoriatic lesional skin is downregulated, the dithranol induced upregulation of the receptor in our model system might help to reveal the therapeutic action of the drug.¶

Key words: Interleukin-8 - Interleukin-10 - Interleukin-10 receptor - Psoriasis - Dithranol 

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Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • Á Farkas
    • 1
  • L. Kemény
    • 1
  • B.J. Szöny
    • 2
  • Zs. Bata-Csörgö
    • 1
  • A. Pivarcsi
    • 1
  • M. Kiss
    • 1
  • M. Széll
    • 3
  • A. Koreck
    • 4
  • A. Dobozy
    • 1
  1. 1.Department of Dermatology, Faculty of General Medicine, University of Szeged, 6701 Szeged, P.O. Box 427 Hungary, Fax: 36 62 545954, e-mail: farkas@derma.szote.u-szeged.huHU
  2. 2.Department of Pathophysiology, Faculty of General Medicine, University of Szeged, 6725 Szeged, Semmelweis u. 1.HU
  3. 3.Dermatological Research Group of the Hungarian Academy of Sciences, 6701 Szeged, P.O. Box 427, Hungary HU
  4. 4.Department of Immunology, University of Medicine and Pharmacy, str. Splaiul Tudor Vladimirescu nr. 14, 1900 Timisoara, Romania RO

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