Inflammation Research

, Volume 50, Issue 1, pp 12–18 | Cite as

Anti-dsDNA antibody up-regulates interleukin 6, but not cyclooxygenase, gene expression in glomerular mesangial cells: a marker of immune-mediated renal damage?

  • C.-L. Yu
  • K.-H. Sun
  • C.-Y. Tsai
  • S.-C. Hsieh
  • H.-S. Yu

Abstract:

Objective and Design: To determine whether anti-double stranded DNA antibody (anti-dsDNA) can affect the synthesis of eicosanoids and cytokines in rat glomerular mesangial cells (RMC).¶Materials or Subjects: Glomerular mesangial cells were isolated and subcultured from Sprague-Dawley rats. Monoclonal anti-dsDNA (12B3 clone) was derived from autoimmune MRL-lpr/lpr mouse by hybridoma technology.¶Methods: The mRNA expression of cyclo-oxygenase type 1 (COX-1), type 2 (COX-2), Th1 (IL-2 and IFN-γ)/Th2 (IL-4 and IL-10) and proinflammatory (IL-6 and TNF-α) and anti-inflammatory (TGF-β) cytokines of RMC ± anti-dsDNA was detected by RT-PCR. The PGE2 production by RMC ± anti-dsDNA was measured by ELISA. The statistical significance was assessed by non-parametric Wilcoxon signed rank test.¶Results: We found RMC spontaneously expressed COX-1, but not COX-2. The incubation of RMC with anti-dsDNA (50ng/ml) did not affect COX expression and PGE2 production by RMC. RMC also spontaneously expressed IL-6, TNF-α and TGF-β mRNA. However, only IL-6 was up-regulated by anti-dsDNA.¶Conclusions: Increased IL-6 expression in RMC may become a marker of anti-dsDNA-mediated immune damage of mesangial cells.¶

Key words: Anti-dsDNA - Rat glomerular mesangial cell - Interleukin-6 - Immune-mediated glomerulonephropathy - Cyclooxygenase 

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Copyright information

© Birkhäuser Verlag, 2001

Authors and Affiliations

  • C.-L. Yu
    • 1
  • K.-H. Sun
    • 2
  • C.-Y. Tsai
    • 3
  • S.-C. Hsieh
    • 4
  • H.-S. Yu
    • 5
  1. 1.Department of Medicine & Institute of Molecular Medicine, National Taiwan University College of Medicine, #7 Chung-Shan South Road, Taipei, Taiwan 100, Fax: 886223957801, e-mail: clyu@ha.mc.ntu.edu.twTW
  2. 2.Faculty of Medical Technology, National Yang-Ming University, 155 Section 2, Lie-Nong Street, Shih Pai Taipei, TaiwanTW
  3. 3.Department of Medicine, Veterans General Hospital-Taipei, Taipei, 201 Section 2, Shih Pai Road, Taipei, TaiwanTW
  4. 4.Department of Medicine, Chang-Hwa Christ Hospital, 135 Non-Shiao Street, Chang-Hwa, TaiwanTW
  5. 5.Department of Dermatology, Kaohsiung Medical College, 100 Section 1, Shu-Cheng Road, Kaohsiung, TaiwanTW

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