Inflammation Research

, Volume 46, Issue 2, pp 72–77

Nitric oxide synthase inhibitor influences prostaglandin and interleukin-1 production in experimental arthritic joints

  • S. B. Verissimo de Mello
  • G. S. Novaes
  • I. M. M. Laurindo
  • M. N. Muscará
  • F. M. de Barros Maciel
  • W. Cossermelli

Abstract.

Objective: To assess involvement of nitric oxide (NO) in the increase in eicosanoid and interleukin- 1 (IL-1) levels in the synovial fluid during antigen-induced arthritis (AIA) in rabbits treated with a competitive inhibitor of NO synthesis. ¶Subjects: Thirteen New Zealand White rabbits were sensitized with 5 mg of methylated bovine serum albumin (mBSA). Arthritis was induced in the knee joint by injecting 0.5 ml of a sterile solution of mBSA (2 mg/ml) into the intra-articular cavity. ¶Treatment: Prior to the induction of arthritis, the animals received N-Omega-Nitro-L-Arginine Methyl Ester (LNAME) or N-Omega-Nitro-D-Arginine Methyl Ester (DNAME) for 2 weeks, both at a dose of 20 mg/kg/day mixed with drinking water. ¶Methods: Leukocyte efflux (total and differential white cell count), vascular permeability (Evans's blue method), synovial PMN cell infiltrate, and total nitrite (NO2.)/nitrate (NO3.) (HPLC), PGE2, TxB2, LTB4 (radioimmunoassay), and IL-1<beta> (ELISA) levels were quantified in the synovial fluid. ¶Results: LNAME but not DNAME significantly suppressed leukocyte efflux and protein leakage into the articular cavity as well as synovial PMN cell infiltrate. Total NO2./NO3., PGE2 and IL-1<beta> levels were significantly reduced in the synovial fluid of LNAME treated animals. TxB2 and LTB4 were not affected by LNAME treatment. ¶Conclusion: These data clearly show NO involvement in the IL-1-induced PGE2 production in the synovial fluid of antigen- induced arthritis in rabbits.

Key words: Experimental arthritis — Nitric oxide — Eicosanoids — Interleukin-1 

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Copyright information

© Birkhäuser Verlag, Basel, 1997

Authors and Affiliations

  • S. B. Verissimo de Mello
    • 1
  • G. S. Novaes
    • 1
  • I. M. M. Laurindo
    • 1
  • M. N. Muscará
    • 2
  • F. M. de Barros Maciel
    • 1
  • W. Cossermelli
    • 1
  1. 1.Rheumatology Division, School of Medicine, University of São Paulo, Av. Dr. Arnaldo, 455, São Paulo, 0124-6903 SP, BrazilBR
  2. 2.Department of Pharmacology, Faculty of Medical Sciences, UNICAMP, P.O. Box 6111, 13081-970, Campinas SP, BrazilBR

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