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Inflammation Research

, Volume 46, Issue 1, pp 19–27 | Cite as

Modulation of tendon healing by nitric oxide

  • G. A. C. Murrell
  • C. Szabo
  • J. A. Hannafin
  •  
  • D. Jang
  • M. M. Dolan
  • X.-H. Deng
  • D. F. Murrell
  • R. F. Warren

Abstract.

Nitric oxide (NO.) is a small, diffusible free radical that is generated from L-arginine by a family of enzymes, collectively termed the nitric oxide synthases. We investigated the role of NO. in tendon healing. NO. synthase activity and immunoreactivity was absent in un-injured rat Achilles tendon. After surgical division there was a five-fold increase in NO. synthase activity and immunoreactivity within the healing tendon at day 7, with a return to near baseline levels at day 14. Inhibition of NO. synthase activity with oral administration of N<omega>-nitro-L-arginine methyl ester (L-NAME) resulted in a significant reduction in cross-sectional area (30% at day 7, p < 0.01, 50% at day 15, p < 0.001) and failure load (24% at day 7, p <0.01) of the healing Achilles tendon constructs. Rats fed the same regimen of the enantiomer of L-NAME, (D-NAME) had normal tendon healing. These results indicate that nitric oxide synthase is induced during tendon healing and inhibition of nitric oxide synthase inhibits this tendon healing.

Key words: Nitric oxide — Tendon healing — Free radical — Wound healing — Nitric oxide synthase 

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Copyright information

© Birkhäuser Verlag, Basel, 1997

Authors and Affiliations

  • G. A. C. Murrell
    • 1
  • C. Szabo
    • 3
  • J. A. Hannafin
    • 1
  •  
  • D. Jang
    • 1
  • M. M. Dolan
    • 1
  • X.-H. Deng
    • 1
  • D. F. Murrell
    • 2
  • R. F. Warren
    • 1
  1. 1.The Laboratory for Soft Tissue Research and Sports Medicine Service, Cornell University Medical College, The Hospital for Special Surgery, 535 East 70th Street, New York, NY 10021, USAUS
  2. 2.Department of Orthopaedic Surgery, University of New South Wales, The St. George Hospital Campus, Kogarah, Sydney, AustraliaAU
  3. 3.Division of Critical Care, Children's Hospital Medical Center, University of Cincinnati, Research Foundation Bldg., 333 Burnet Ave, Cincinnati, OH 45229, USAUS

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