Malt1 inactivation attenuates experimental colitis through the regulation of Th17 and Th1/17 cells
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Objective and design
Protease activity of MALT lymphoma-translocation protein 1 (Malt1) plays an important role in the development of colitis, but the detailed mechanism has not been fully elucidated.
Effects of Malt1 protease on the activation of T cells and the development of experimental colitis was investigated using Malt1 protease-deficient (PD) mouse.
IL-2 production from CD4+ T cells of Malt1 PD mice was decreased compared with that of wild-type (WT) mice. Intraperitoneal injection of anti-CD3 antibody into Malt1 PD mouse induced less productions of IL-17 in the plasma, as well as the colonic gene expression of IL-17A, compared with WT mice, whereas IFN-γ production was not impaired. In naïve T-cell transfer colitis model, Malt1 PD T cells induced less disease severity than WT T cells. Then, reduction in the populations of Th17 and Th1/17 cells was observed in the mesenteric lymph nodes of the recipient mice transferred with Malt1 PD T cells, whereas those of Th1 cells were not impaired. IL-17A expression in the colon was also decreased in the mouse receiving Malt1 PD T cells.
Inactivation of Malt1 protease activity abrogates Th17 and Th1/17 cell activation, resulting in the amelioration of experimental colitis.
KeywordsMalt1 Experimental colitis Th17 cells Th1/17 cells IL-17
We thank the following employees of Takeda Pharmaceutical Company Limited, Naoko Matsunaga and Takeo Arita, for contributing to discussion on in vivo experiment, Hikaru Saito for contributing to in vivo experiment, and Kimihiko Iwachidow, Takashi Yano, and Yuki Nakamura for contributing to generation, breeding and supply of Malt1 PD mouse.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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