Inflammation Research

, Volume 67, Issue 11–12, pp 903–911 | Cite as

Nuciferine alleviates LPS-induced mastitis in mice via suppressing the TLR4-NF-κB signaling pathway

  • Xingxing Chen
  • Xintian Zheng
  • Min Zhang
  • Huifang Yin
  • Kangfeng Jiang
  • Haichong Wu
  • Ailing DaiEmail author
  • Shoushen YangEmail author
Original Research Paper



Nuciferine, a major bioactive component from the lotus leaf, has been reported to have notable anti-inflammatory activities such as renal inflammation and acute lung injury in previous studies. Mastitis is one of the most prevalent diseases in the dairy cattle, which causes large economic losses for the dairy industry. However, the effects of nuciferine on lipopolysaccharide (LPS)-induced mastitis have not been reported.

Methods and results

Here, we investigated the anti-inflammatory effects of nuciferine on LPS-induced mastitis in mice and illuminated its potential mechanism on the TLR4-mediated signaling pathway in mouse mammary epithelial cells (mMECs). Histopathological changes and myeloperoxidase (MPO) activity assay showed that nuciferine treatment significantly alleviated the LPS-induced injury of mammary gland flocculus, inflammatory cells infiltration. qPCR and ELISA assays indicated that nuciferine dose-dependently reduced the levels of TNF-α and IL-1β, which indicated that nuciferine might have therapeutic effects on mastitis. Furthermore, nuciferine treatment significantly decreased the expression of TLR4 in a dose-dependent manner. Besides, nuciferine was also found to suppress LPS-induced NF-κB activation.


These findings indicate that nuciferine potently ameliorates LPS-induced mastitis by inhibition of the TLR4-NF-κB signaling pathway.


Nuciferine LPS Mastitis Anti-inflammation TLR4 NF-κB 



Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology (No. ZDSYS2017005).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Xingxing Chen
    • 1
    • 2
  • Xintian Zheng
    • 1
    • 2
  • Min Zhang
    • 1
    • 2
  • Huifang Yin
    • 1
    • 2
  • Kangfeng Jiang
    • 3
  • Haichong Wu
    • 2
    • 3
  • Ailing Dai
    • 1
    • 2
    Email author
  • Shoushen Yang
    • 1
    • 2
    Email author
  1. 1.College of Life Sciences of Longyan UniversityLongyanPeople’s Republic of China
  2. 2.Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and BiotechnologyLongyanPeople’s Republic of China
  3. 3.Department of Clinical Veterinary Medicine, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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