Expression analysis of toll-like receptors of Dengue-infected cornea by real-time polymerase chain reaction

  • Durgadevi Parthasarathy
  • Janani Krishnan Madhuravasal
  • Padmapriya Jayavel
  • Lily Therese Kulandai
  • Madhavan Hajib Narahari Rao
  • Malathi Jambulingam
Short Communication
  • 34 Downloads

Abstract

Background

Toll-like receptors (TLRs) play a significant role based on innate immune mechanism during viral infection. TLR signaling mechanism designates to protect the cells from invading viruses. The expression of TLRs during dengue virus (DENV) infection not yet well explained. This study evaluates the TLR gene expression from DENV-infected patient’s cornea.

Methods

Reverse transcriptase PCR was performed for the detection and genotyping of viral nucleic acid from corneal grafts and DENV-infected cell suspension. TLR expression studies were done on DENV-infected cornea by real-time RT2 Profiler PCR Array.

Results

The reverse transcriptase PCR and genotyping confirmed the presence of DENV-3. TLR expression studies revealed the upregulated expression of TLR4, TLR7, TLR9 and TLR10.

Conclusion

Molecular testing of DENV reveals that serological positivity induces transmission of the virus through cornea and stimulates the expression of TLR4, TLR7, TLR9 and TLR10, which may lead to up-regulation of innate pro-inflammatory response in the cornea.

Keywords

Dengue virus Toll-like receptor RT2 profiler PCR array Reverse transcriptase polymerase chain reaction Genotyping 

Notes

Acknowledgements

This work is not supported by any funding agency.

Compliance with ethical standards

Conflict of interest

The authors have declared that there is no conflict of interest.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Durgadevi Parthasarathy
    • 1
  • Janani Krishnan Madhuravasal
    • 1
  • Padmapriya Jayavel
    • 1
  • Lily Therese Kulandai
    • 1
  • Madhavan Hajib Narahari Rao
    • 1
  • Malathi Jambulingam
    • 1
  1. 1.Larsen & Toubro Microbiology Research CentreVision Research FoundationChennaiIndia

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