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Mesenchymal stem cells cannot affect mRNA expression of toll-like receptors in different tissues during sepsis

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Abstract

Objective and design

Experimental animal models and human clinical studies support a crucial role for TLRs in infectious diseases. The aim of this study was to test the ability of MSCs, which have immunomodulatory effects, of altering the mRNA expression of toll-like receptors during a experimental model of sepsis in different tissues.

Materials and methods

Three experimental groups (male C57BL/6 mice) were formed for the test: control group, untreated septic group and septic group treated with MSCs (1 × 106 cells/animal). Lungs, cortex, kidney, liver and colon tissue were dissected after 12 h of sepsis induction and TLR2/3/4/9 mRNA were evaluated by RT-qPCR.

Results

We observed a decrease of TLR2 and 9 mRNA expression in the liver of the sepsis group, while TLR3 was decreased in the lung and liver. No change was found between the sepsis group and the sepsis + MSC group.

Conclusions

In this model of experimental sepsis the MSCs were unable to modify the mRNA expression of the different toll-like receptors evaluated.

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Authors and Affiliations

  1. Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil

    Leonardo Pedrazza, Fernanda Bordignon Nunes & Jarbas Rodrigues de Oliveira

  2. Laboratório de Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil

    Talita Carneiro Brandão Pereira & Maurício Reis Bogo

  3. Programa de Graduação de Biotecnologia, Centro Universitário Univates, Lajeado, Rio Grande do Sul, CEP 95900-000, Brazil

    Ana Lucia Abujamra

Authors
  1. Leonardo Pedrazza
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  2. Talita Carneiro Brandão Pereira
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  3. Ana Lucia Abujamra
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  4. Fernanda Bordignon Nunes
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  5. Maurício Reis Bogo
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  6. Jarbas Rodrigues de Oliveira
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Corresponding author

Correspondence to Leonardo Pedrazza.

Additional information

Responsible Editor: John Di Battista.

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Pedrazza, L., Pereira, T.C.B., Abujamra, A.L. et al. Mesenchymal stem cells cannot affect mRNA expression of toll-like receptors in different tissues during sepsis. Inflamm. Res. 66, 547–551 (2017). https://doi.org/10.1007/s00011-017-1042-6

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  • DOI: https://doi.org/10.1007/s00011-017-1042-6

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