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Inflammation Research

, Volume 62, Issue 12, pp 1073–1078 | Cite as

Direct comparison of laser Doppler flowmetry and laser Doppler imaging for assessment of experimentally-induced inflammation in human skin

  • Lars J. Petersen
Original Research Paper

Abstract

Objective and design

Laser Doppler imaging (LDI) and laser Doppler flowmetry (LDF) can measure localized skin perfusion. The purpose of the study was to directly compare LDF with LDI as a tool for measuring skin blood changes in an experimental model of chemically-induced skin inflammation.

Methods

Regions of interest 1.8 cm2 in area on the forearm skin of eight healthy volunteers were randomized and exposed to 0.25, 0.5, 1, or 2 % topical sodium lauryl sulfate (SLS) or vehicle for 24 h. Mean blood flow was measured by LDI and LDF at 24, 48, and 72 h. Inflammation was clinically graded using a standardized, clinical score.

Results

Sodium lauryl sulfate induced significant, dose-dependent local inflammation. Both Doppler methods were significantly correlated with the clinical grading (LDF, r = 0.755; LDI, r = 0.836). LDF and LDI showed similar significance differences with regard to dose- and time-response patterns compared to the vehicle. The absolute and relative LDF and LDI values were significantly correlated.

Conclusions

Laser Doppler flowmetry and LDI showed similar dose- and time-response relations in irritant-induced inflammatory skin reactions. For the assessment of localized skin reactions, LDI possesses no apparent advantages over the less expensive LDF method for grading dermal inflammatory reactions.

Keywords

Comparative study Dose-response relation Human Inflammation Laser Doppler flowmetry Laser Doppler imaging Reproducibility Skin irritants Sodium lauryl sulfate 

Notes

Acknowledgments

The study was supported by a research grant from the Rosa and Asta Jensen Foundation.

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Copyright information

© Springer Basel 2013

Authors and Affiliations

  1. 1.Department of Nuclear MedicineAalborg University HospitalAalborgDenmark
  2. 2.Department of Clinical MedicineAalborg UniversityAalborgDenmark

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