Direct comparison of laser Doppler flowmetry and laser Doppler imaging for assessment of experimentally-induced inflammation in human skin
- 378 Downloads
Objective and design
Laser Doppler imaging (LDI) and laser Doppler flowmetry (LDF) can measure localized skin perfusion. The purpose of the study was to directly compare LDF with LDI as a tool for measuring skin blood changes in an experimental model of chemically-induced skin inflammation.
Regions of interest 1.8 cm2 in area on the forearm skin of eight healthy volunteers were randomized and exposed to 0.25, 0.5, 1, or 2 % topical sodium lauryl sulfate (SLS) or vehicle for 24 h. Mean blood flow was measured by LDI and LDF at 24, 48, and 72 h. Inflammation was clinically graded using a standardized, clinical score.
Sodium lauryl sulfate induced significant, dose-dependent local inflammation. Both Doppler methods were significantly correlated with the clinical grading (LDF, r = 0.755; LDI, r = 0.836). LDF and LDI showed similar significance differences with regard to dose- and time-response patterns compared to the vehicle. The absolute and relative LDF and LDI values were significantly correlated.
Laser Doppler flowmetry and LDI showed similar dose- and time-response relations in irritant-induced inflammatory skin reactions. For the assessment of localized skin reactions, LDI possesses no apparent advantages over the less expensive LDF method for grading dermal inflammatory reactions.
KeywordsComparative study Dose-response relation Human Inflammation Laser Doppler flowmetry Laser Doppler imaging Reproducibility Skin irritants Sodium lauryl sulfate
The study was supported by a research grant from the Rosa and Asta Jensen Foundation.
- 7.Opazo Saez AM, Mosel F, Nurnberger J, Rushentsova U, Gossl M, Mitchell A, Schafers RF, Philipp T, Wenzel RR. Laser Doppler imager (LDI) scanner and intradermal injection for in vivo pharmacology in human skin microcirculation: responses to acetylcholine, endothelin-1 and their repeatability. Br J Clin Pharmacol. 2005;59:511–9.PubMedCrossRefGoogle Scholar
- 13.Broby-Johansen U, Karlsmark T, Petersen LJ, Serup J. Ranking of the antipsoriatic effect of various topical corticosteroids applied under a hydrocolloid dressing—skin-thickness, blood-flow and colour measurements compared to clinical assessments. Clin Exp Dermatol. 1990;15:343–8.PubMedCrossRefGoogle Scholar
- 14.Henricson J, Tesselaar E, Persson K, Nilsson G, Sjoberg F. Assessment of microvascular function by study of the dose-response effects of iontophoretically applied drugs (acetylcholine and sodium nitroprusside)—methods and comparison with in vitro studies. Microvasc Res. 2007;73:143–9.PubMedCrossRefGoogle Scholar
- 18.Fullerton A, Stucker M, Wilhelm KP, Wardell K, Anderson C, Fischer T, Nilsson GE, Serup J. Guidelines for visualization of cutaneous blood flow by laser Doppler perfusion imaging: a report from the Standardization Group of the European Society of Contact Dermatitis based upon the HIRELADO European community project. Contact Dermat. 2002;46:129–40.CrossRefGoogle Scholar