Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines
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Paeoniflorin (Pae) was previously reported to inhibit inflammation in the skin of mice with allergic contact dermatitis (ACD); however, the mechanism remains unclear. The primary purpose of this study was to investigate the effect of Pae on the regulation of cytokine production in a murine model of ACD.
ACD was induced in the mice by repeated application of dinitrochlorobenzene (DNCB) to their skin. Cutaneous inflammation was evaluated by measuring ear swelling and by histological examination. The cytokine levels were measured by enzyme-linked immunosorbent assays.
The results showed that topical application of DNCB caused obvious swelling and inflammatory cell infiltration. Treatment with Pae (70 or 140 mg/kg/d) significantly inhibited the cutaneous inflammation and decreased thymocyte proliferation in the mice with ACD. Additional data indicated that Pae increased interleukin-4 (IL-4) and IL-10 production but reduced IL-2 and IL-17 levels in the serum as well as in thymocyte and splenocyte culture supernatants. As expected, IL-2 and IL-17 levels in the serum displayed a significant positive correlation with the severity of skin inflammation. In contrast, IL-4 and IL-10 levels were negatively correlated with the inflammation.
The anti-inflammatory action of Pae in the murine model of ACD may be related to its regulation of an imbalanced cytokine production.
KeywordsAllergic contact dermatitis Paeoniflorin Interleukin-2 Interleukin-4 Interleukin-10 Interleukin -17
This work was supported by the National Natural Science Foundation of China (No. 30572356, No. 81173075). The authors would like thank Pei-pei Li, Qiong Qin, Feng Yang, Guo-xiong Xie, Wei He, Chen Chen and Shan-shan Song for their excellent technical assistance.
Conflict of interest
There is no conflict of interest to declare.
- 11.Min WQ, Wei Q, Li HY, et al. A clinical study of total glucosides paeony in the treatment of rheumatoid arthritis: a multi-center trial. Chin J Rheumatol. 2005;9:487–91.Google Scholar
- 22.Xu H, DiIulio NA, Fairchild RL. T cell populations primed by hapten sensitization in contact sensitivity are distinguished by polarized patterns of cytokine production: interferon gamma- producing (Tc1) effector CD8+ T cells and interleukin (IL) 4/IL-10-producing (Th2) negative regulatory CD4+ T cells. Exp Med. 1996;183:1001–12.CrossRefGoogle Scholar