Inflammation Research

, Volume 62, Issue 12, pp 1035–1044 | Cite as

Paeoniflorin inhibits inflammatory responses in mice with allergic contact dermatitis by regulating the balance between inflammatory and anti-inflammatory cytokines

  • Chun Wang
  • Jun Yuan
  • Hua-xun Wu
  • Yan Chang
  • Qing-tong Wang
  • Yu-jing Wu
  • Li-hua Liu
  • Wei WeiEmail author
Original Research Paper



Paeoniflorin (Pae) was previously reported to inhibit inflammation in the skin of mice with allergic contact dermatitis (ACD); however, the mechanism remains unclear. The primary purpose of this study was to investigate the effect of Pae on the regulation of cytokine production in a murine model of ACD.


ACD was induced in the mice by repeated application of dinitrochlorobenzene (DNCB) to their skin. Cutaneous inflammation was evaluated by measuring ear swelling and by histological examination. The cytokine levels were measured by enzyme-linked immunosorbent assays.


The results showed that topical application of DNCB caused obvious swelling and inflammatory cell infiltration. Treatment with Pae (70 or 140 mg/kg/d) significantly inhibited the cutaneous inflammation and decreased thymocyte proliferation in the mice with ACD. Additional data indicated that Pae increased interleukin-4 (IL-4) and IL-10 production but reduced IL-2 and IL-17 levels in the serum as well as in thymocyte and splenocyte culture supernatants. As expected, IL-2 and IL-17 levels in the serum displayed a significant positive correlation with the severity of skin inflammation. In contrast, IL-4 and IL-10 levels were negatively correlated with the inflammation.


The anti-inflammatory action of Pae in the murine model of ACD may be related to its regulation of an imbalanced cytokine production.


Allergic contact dermatitis Paeoniflorin Interleukin-2 Interleukin-4 Interleukin-10 Interleukin -17 



This work was supported by the National Natural Science Foundation of China (No. 30572356, No. 81173075). The authors would like thank Pei-pei Li, Qiong Qin, Feng Yang, Guo-xiong Xie, Wei He, Chen Chen and Shan-shan Song for their excellent technical assistance.

Conflict of interest

There is no conflict of interest to declare.

Supplementary material

11_2013_662_MOESM1_ESM.pdf (110 kb)
Supplementary material 1 (PDF 109 kb)


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Copyright information

© Springer Basel 2013

Authors and Affiliations

  • Chun Wang
    • 1
  • Jun Yuan
    • 1
  • Hua-xun Wu
    • 1
  • Yan Chang
    • 1
  • Qing-tong Wang
    • 1
  • Yu-jing Wu
    • 1
  • Li-hua Liu
    • 1
  • Wei Wei
    • 1
    Email author
  1. 1.Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of EducationHefeiChina

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