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The anti-inflammatory action of Bothrops jararaca snake antithrombin on acute inflammation induced by carrageenan in mice

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Abstract

Objective and design

Antithrombin is known as the most important natural coagulation inhibitor and has been shown to have anti-inflammatory properties. The present study aimed to investigate the effects of Bothrops jararaca antithrombin on acute inflammation induced by carrageenan in mice.

Methods

We evaluated the anti-inflammatory activity of antithrombin on models of paw edema formation, cell migration and leukocyte–endothelium interaction in mice (Swiss; n = 5). Acute inflammation was induced by the administration of carrageenan (15 mg kg−1).

Results

Treatment with B. jararaca antithrombin (1 mg kg−1) 1 h before or after carrageenan administration significantly inhibited paw edema formation, reduced cell influx to the peritoneal cavity due to reduction in the migration of polymorphonuclear cells, and attenuated leukocyte rolling in the microcirculation of the cremaster muscle.The effects of antithrombin on vascular and cellular events of inflammation were completely abolished by treatment with the cyclo-oxygenase inhibitor indomethacin (4 mg kg−1), suggesting the involvement of prostacyclin in the mechanism of inflammation inhibition by B. jararaca antithrombin.

Conclusion

This work showed for the first time the anti-inflammatory properties of B. jararaca antithrombin on vascular and cellular events of inflammation. These findings suggest that antithrombin is effective in preventing paw edema formation, cell migration and leukocyte rolling induced by carrageenan in mice.

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Acknowledgments

We acknowledge the Mass Spectrometry Laboratory at Brazilian Biosciences National Laboratory, CNPEM-ABTLuS, Campinas, Brazil for their support with the mass spectrometry analysis. This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (09/50199-2, 09/03484-3, 11/21558-4) and Conselho Nacional de Desenvolvimento Científico e Tecnológico.

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Authors and Affiliations

  1. Laboratório de Herpetologia, Instituto Butantan, Avenida Vital Brazil, 1500, CEP 05503-900, São Paulo, Brazil

    Karen de Morais-Zani, Jacilene Barbosa da Silva, Kathleen Fernandes Grego & Anita Mitico Tanaka-Azevedo

  2. Programa de Pós-Graduação Interunidades em Biotecnologia, Instituto de Pesquisas Tecnológicas, Instituto Butantan, Universidade de São Paulo, Avenida Professor Lineu Prestes, 2415, CEP 05508-900, São Paulo, Brazil

    Karen de Morais-Zani & Anita Mitico Tanaka-Azevedo

  3. Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 321, CEP 05508-900, São Paulo, Brazil

    Fernanda Peixoto Barbosa Nunes

  4. Laboratório Especial de Toxinolgia Aplicada, CEPID/FAPESP, Instituto Butantan, Avenida Vital Brazil, 1500, CEP 05503-900, São Paulo, Brazil

    Márcio José Ferreira & Mônica Lopes-Ferreira

  5. Departamento de Bioquímica, Universidade Federal de São Paulo, Rua Três de Maio, 100, CEP 04044-020, São Paulo, Brazil

    Aparecida Sadae Tanaka

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  1. Karen de Morais-Zani
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  2. Fernanda Peixoto Barbosa Nunes
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  3. Jacilene Barbosa da Silva
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  4. Márcio José Ferreira
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  6. Mônica Lopes-Ferreira
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  7. Aparecida Sadae Tanaka
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  8. Anita Mitico Tanaka-Azevedo
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Correspondence to Anita Mitico Tanaka-Azevedo.

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de Morais-Zani, K., Nunes, F.P.B., da Silva, J.B. et al. The anti-inflammatory action of Bothrops jararaca snake antithrombin on acute inflammation induced by carrageenan in mice. Inflamm. Res. 62, 733–742 (2013). https://doi.org/10.1007/s00011-013-0628-x

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  • DOI: https://doi.org/10.1007/s00011-013-0628-x

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