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Inflammation Research

, Volume 61, Issue 4, pp 359–365 | Cite as

Functional polymorphisms in XRCC-1 and APE-1 contribute to increased apoptosis and risk of ulcerative colitis

  • Avinash Bardia
  • Santosh K. Tiwari
  • Sivaram Gunisetty
  • Farha Anjum
  • Pratibha Nallari
  • Md. Aejaz Habeeb
  • Aleem A. Khan
Original Research Paper

Abstract

Objective

The present study was designed to investigate the role of X-ray cross-complementing group 1 (XRCC1) and apurinic/apyrimidinic endonuclease 1 (APE1) polymorphisms in apoptosis and the risk of ulcerative colitis (UC).

Materials and methods

Blood samples from 384 unrelated subject (age range 18–65 years; 171 with UC, 213 healthy controls) were collected after colonoscopy. Genomic DNA was isolated and genotyped for XRCC1 Arg399Gln and APE1 Asp148Glu using a confronting two-pair primers polymerase chain reaction. Apoptosis and intracellular reactive oxygen species (ROS) levels in peripheral blood mononuclear cells were measured using annexin-V and H2DCFDA assay, respectively.

Results

The frequency of genotype Arg399Gln (heterozygous) of XRCC1 gene was significantly higher in patients with UC than the controls (odds ratio [OR] 1.73; 95% confidence interval [CI] 1.13–2.64; p = 0.01). Similarly the genotypic frequency of APE1 Asp148Glu showed statistically significant incidence among UC subjects (OR 1.54; 95% CI 1.02–2.33; p = 0.04). Polymorphism in XRCC1 Arg399Gln and APE1 Asp148Glu together considerably increased the risk of UC (OR 2.303; 95% CI 1.43–3.69; p = 0.0007). ROS levels were high in UC subjects compared with controls (p = 0.01).

Conclusion

Polymorphisms in XRCC1 Arg399Gln and APE1 Asp148Glu significantly increased the rate of apoptosis and risk of ulcerative colitis.

Keywords

Ulcerative colitis X-ray cross-complementing group 1 Apurinic/apyrimidinic endonuclease 1 Apoptosis Reactive oxygen species 

Notes

Acknowledgments

We would like to thank Mrs. Deepika, Research Fellow, Department of Genetics, Osmania University for statistical analysis. The authors would also like to acknowledge Mr. Pinaki Ghosh and Mr. Arvind Ghule, Senior Research Fellows, Pune College of Pharmacy, Pune, for sharing ApoAlert™ Annexin V Apoptosis Kit for our experiments. The study was not supported by any intra- or extramural funds.

Conflict of interest

None.

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Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  • Avinash Bardia
    • 1
  • Santosh K. Tiwari
    • 1
  • Sivaram Gunisetty
    • 1
  • Farha Anjum
    • 1
  • Pratibha Nallari
    • 2
  • Md. Aejaz Habeeb
    • 1
  • Aleem A. Khan
    • 1
  1. 1.Centre for Liver Research and DiagnosticsDeccan College of Medical SciencesHyderabadIndia
  2. 2.Department of GeneticsOsmania UniversityHyderabadIndia

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