Inflammation Research

, Volume 61, Issue 3, pp 189–196 | Cite as

Annexin A1 protein attenuates cyclosporine-induced renal hemodynamics changes and macrophage infiltration in rats

  • Leandro Pires Araujo
  • Renata Ramos Truzzi
  • Gloria Elisa Florido Mendes
  • Marcus Alexandre Mendes Luz
  • Emmanuel A. Burdmann
  • Sonia Maria OlianiEmail author
Original Research Paper



Cyclosporine (CsA) remains an important immunosuppressant for transplantation and for treatment of autoimmune diseases. The most troublesome side effect of CsA is renal injury. Acute CsA-induced nephrotoxicity is characterized by reduced renal blood flow (RBF) and glomerular filtration rate (GFR) due to afferent arteriole vasoconstriction. Annexin A1 (ANXA1) is a potent anti-inflammatory protein with protective effect in renal ischemia/reperfusion injury. Here we study the effects of ANXA1 treatment in an experimental model of acute CsA nephrotoxicity.


Salt-depleted rats were randomized to treatment with VH (vehicles 1 mL/kg body weight/day), ANXA1 (Ac2-26 peptide 1 mg/kg body weight/day intraperitoneally), CsA (20 mg/kg body weight/day subcutaneously) and CsA + ANXA1 (combination) for seven days. We compared renal function and hemodynamics, renal histopathology, renal tissue macrophage infiltration and renal ANXA1 expression between the four groups.


CsA significantly impaired GFR and RBF, caused tubular dilation and macrophage infiltration and increased ANXA1 renal tissue expression. Treatment with ANXA1 attenuated CSA-induced hemodynamic changes, tubular injury and macrophage infiltration.


ANXA1 treatment attenuated renal hemodynamic injury and inflammation in an acute CsA nephrotoxicity model.


Acute renal injury Annexin A1 Cyclosporine nephrotoxicity Immunosuppression Inflammation 



Fundação de Amparo à Pesquisa do Estado de São Paulo - FAPESP (Grant 2008/01,048-9 to L.P.A.); Conselho Nacional de Desenvolvimento Científico e Tecnológico–CNPq (Grant 306,074/2007-9 to S.M.O. and 307,371/2006-9 to E.A.B.).

Conflict of interest

The authors have no competing financial interests to disclose in relation to this manuscript.


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Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  • Leandro Pires Araujo
    • 1
  • Renata Ramos Truzzi
    • 2
  • Gloria Elisa Florido Mendes
    • 3
  • Marcus Alexandre Mendes Luz
    • 3
  • Emmanuel A. Burdmann
    • 3
    • 4
  • Sonia Maria Oliani
    • 1
    • 2
    Email author
  1. 1.Post-Graduation Program in MorphologyFederal University of São Paulo (UNIFESP)São PauloBrazil
  2. 2.Department of BiologyInstituto de Biociências, Letras e Ciências Exatas (IBILCE), São Paulo State University (UNESP)São PauloBrazil
  3. 3.Division of NephrologySão José do Rio Preto Medical SchoolSão PauloBrazil
  4. 4.Division of NephrologyUniversity of São Paulo Medical SchoolSão PauloBrazil

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