Inflammation Research

, Volume 60, Issue 6, pp 521–523 | Cite as

Adjuvant arthritis influences expression of housekeeping genes

Short Communication
First Online:
Received:
Accepted:

Abstract

Objective and method

We determined whether adjuvant arthritis (AA) affects expression of three housekeeping (HK) genes, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), β-actin and 18s ribosomal RNA (18s rRNA) in organs of Sprague–Dawley rats using the real-time reverse-transcription polymerase chain reaction (RT-PCR).

Results

Expression of 18s rRNA was unaffected by AA whereas that of β-actin was reduced by 52, 62 and 81% in the liver, kidney and intestine, respectively, but not in the heart. AA significantly reduced GAPDH expression in the intestine.

Conclusion

For reliable results, the HK gene must be carefully selected.

Keywords

Polymerase chain reaction Adjuvant arthritis HK genes 
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Notes

Acknowledgments

SH is a recipient of an Egyptian Government scholarship. The study was supported by a grant from the Canadian Institute of Health Research.

Conflict of interest

None.

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Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  1. 1.Faculty of Pharmacy and Pharmaceutical SciencesUniversity of AlbertaEdmontonCanada

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