Inflammation Research

, Volume 60, Issue 6, pp 597–604

Reactive oxygen species and NADPH oxidase 4 induced by transforming growth factor β1 are the therapeutic targets of polyenylphosphatidylcholine in the suppression of human hepatic stellate cell activation

  • Remina Ikeda
  • Kyoko Ishii
  • Yoshiko Hoshikawa
  • Junya Azumi
  • Yuta Arakaki
  • Toshihiro Yasui
  • Shizuka Matsuura
  • Yoshiaki Matsumi
  • Yohei Kono
  • Yusuke Mizuta
  • Akihiro Kurimasa
  • Ichiro Hisatome
  • Scott L. Friedman
  • Hironaka Kawasaki
  • Goshi Shiota
Original Research Paper

DOI: 10.1007/s00011-011-0309-6

Cite this article as:
Ikeda, R., Ishii, K., Hoshikawa, Y. et al. Inflamm. Res. (2011) 60: 597. doi:10.1007/s00011-011-0309-6

Abstract

Objective and design

To clarify the molecular mechanism of polyenylphosphatidylcholine (PPC), we examined the involvement of reactive oxygen species (ROS) and NADPH oxidase 4 (Nox4) in human hepatic stellate cells (HSCs).

Material

Using human LX-2 HSC cells, we examined the effects of PPC on expression of α-smooth muscle actin (α-SMA) and collagen 1, generation of ROS, Nox4 expression, p38 activation and cell proliferation, induced by transforming growth factor β1 (TGFβ1).

Results

PPC suppressed ROS which are induced by TGFβ1, phosphorylation of p38MAPK, and expression levels of α-SMA and collagen 1 in a dose-dependent manner. Higher concentrations of PPC also suppressed Nox4 levels.

Conclusion

These results suggest that ROS and Nox4 induced by TGFβ1 are the therapeutic targets of PPC in the suppression of human hepatic stellate cell activation.

Keywords

Polyenylphosphatidylcholine NADPH oxidase 4 Hepatic stellate cells Oxidative stress 

Abbreviations

PPC

Polyenylphosphatidylcholine

HSC

Hepatic stellate cell

Nox

NADPH oxidase

MFB

Myofibroblast

PDGF

Platelet-derived growth factor

TGFβ1

Transforming growth factor β-1

ECM

Extracellular matrix

HCC

Hepatocellular carcinoma

LC

Liver cirrhosis

Copyright information

© Springer Basel AG 2011

Authors and Affiliations

  • Remina Ikeda
    • 1
  • Kyoko Ishii
    • 1
  • Yoshiko Hoshikawa
    • 1
  • Junya Azumi
    • 1
  • Yuta Arakaki
    • 1
  • Toshihiro Yasui
    • 1
  • Shizuka Matsuura
    • 1
  • Yoshiaki Matsumi
    • 1
  • Yohei Kono
    • 1
  • Yusuke Mizuta
    • 1
  • Akihiro Kurimasa
    • 1
  • Ichiro Hisatome
    • 2
  • Scott L. Friedman
    • 3
  • Hironaka Kawasaki
    • 4
  • Goshi Shiota
    • 1
  1. 1.Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of MedicineTottori UniversityYonagoJapan
  2. 2.Division of Regenerative Therapeutics, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of MedicineTottori UniversityYonagoJapan
  3. 3.Division of Liver DiseasesMount Sinai School of MedicineNew YorkUSA
  4. 4.Department of Internal MedicineSan-in Rosai HospitalYonagoJapan

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