The effects of lazaroid U-74389G in a rat sepsis model
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Objective and design
To examine the protective effects of a lazaroid, 21-aminosteroid U-74389G, in a rat septic shock model.
Materials or subjects
Male Sprague–Dawley rats (n = 60) aged 6–8 months.
Groups were exposed to 500 cGy radiation followed by E. coli inoculation, and either placebo or lazaroid injection (10 mg/kg intraperitoneal) 5 days after irradiation.
Hemodynamic measurements, arterial blood gases, serum lactate, total antioxidative capacity, and cytokine levels were measured at specific time intervals.
Treatment with the lazaroid U-74389G maintained cardiac output and mean aortic pressure. Lazaroid treatment also prevented the increase in serum lactate seen in placebo-treated rats. Cytokine serum levels in lazaroid-treated rats were not significantly different from those in placebo-treated rats at any time point.
Lazaroid treatment of E. coli-inoculated septic animals lessens the hemodynamic deterioration seen in sepsis.
KeywordsSeptic shock Cardiac output Lactate Cytokines E. coli
We thank Chi-Huei Chang, Hsin-Hui Tseng, and Yang-Ming Tseng for contributing animal care, linear accelerator operation, and laboratory examinations. The grant was supported by Kaohsiung Veterans General Hospital, VGHKS 95-064.