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Inflammation Research

, Volume 60, Issue 1, pp 29–35 | Cite as

The effects of lazaroid U-74389G in a rat sepsis model

  • Yun-Te Chang
  • Shue-Ren Wann
  • Kai-Hseng Hsieh
  • Yung-Ching Liu
  • Ching-Hsiung Chang
  • Mu-Shun Huang
  • Chun-I Huang
  • Hong-Tai Chang
Original Research Paper

Abstract

Objective and design

To examine the protective effects of a lazaroid, 21-aminosteroid U-74389G, in a rat septic shock model.

Materials or subjects

Male Sprague–Dawley rats (n = 60) aged 6–8 months.

Treatment

Groups were exposed to 500 cGy radiation followed by E. coli inoculation, and either placebo or lazaroid injection (10 mg/kg intraperitoneal) 5 days after irradiation.

Methods

Hemodynamic measurements, arterial blood gases, serum lactate, total antioxidative capacity, and cytokine levels were measured at specific time intervals.

Results

Treatment with the lazaroid U-74389G maintained cardiac output and mean aortic pressure. Lazaroid treatment also prevented the increase in serum lactate seen in placebo-treated rats. Cytokine serum levels in lazaroid-treated rats were not significantly different from those in placebo-treated rats at any time point.

Conclusions

Lazaroid treatment of E. coli-inoculated septic animals lessens the hemodynamic deterioration seen in sepsis.

Keywords

Septic shock Cardiac output Lactate Cytokines E. coli 

Notes

Acknowledgments

We thank Chi-Huei Chang, Hsin-Hui Tseng, and Yang-Ming Tseng for contributing animal care, linear accelerator operation, and laboratory examinations. The grant was supported by Kaohsiung Veterans General Hospital, VGHKS 95-064.

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Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • Yun-Te Chang
    • 1
    • 5
  • Shue-Ren Wann
    • 1
  • Kai-Hseng Hsieh
    • 2
  • Yung-Ching Liu
    • 3
  • Ching-Hsiung Chang
    • 4
  • Mu-Shun Huang
    • 5
  • Chun-I Huang
    • 5
  • Hong-Tai Chang
    • 1
  1. 1.Department of Emergency MedicineKaohsiung Veterans General HospitalKaohsiung CityTaiwan
  2. 2.Department of PediatricsKaohsiung Veterans General HospitalKaohsiungTaiwan
  3. 3.The Infectious Diseases SectionKaohsiung Veterans General HospitalKaohsiungTaiwan
  4. 4.Department of Radiation OncologyKaohsiung Veterans General HospitalKaohsiungTaiwan
  5. 5.School of MedicineNational Yang-Ming UniversityTaipeiTaiwan

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