Diacerein decreases TNF-α and IL-1β levels in peritoneal fluid and prevents Baker’s yeast-induced fever in young rats
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To investigate the effect of diacerein, an anti-inflammatory drug, on body temperature and protocols of fever induction in male Wistar rats.
The effect of diacerein (5.0 mg/kg, s.c.) on rectal temperature (T R) changes induced by Baker’s yeast (0.135 g/kg, i.p.) and PGE2 (10 ng/animal, i.t.) was evaluated. T R changes were recorded over time. The leukocyte count and TNF-α and IL-1β content were evaluated in the peritoneal fluid by means of optical microscopy and enzyme immunoassay (ELISA kits), respectively.
The administration of diacerein to febrile animals attenuated Baker’s yeast-induced fever but did not alter prostaglandin E2-induced fever. Diacerein prevented the development of Baker’s yeast-induced fever and significantly attenuated the increase in peritoneal leukocytes and decreased IL-1β and TNF-α levels in peritoneal fluid.
These data suggest that diacerein partially protects against Baker’s yeast-induced fever and peritoneal leukocyte migration, and indicate that this effect appears to be due to inhibition of release of cytokines (such as TNF-α and IL-1β).
KeywordsFever Cytokines Leukocyte migration In vivo inflammation Infection Baker’s yeast
The authors thank Paulino Aguiar and Florindo Duarte for competent technical support. Research supported by CNPq, CAPES and FAPERGS. C.F.M. and M.A.R. are the recipients of CNPq fellowships, grant numbers 500120/2003-0 and 500096/2003-1, respectively.
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