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Inflammation Research

, Volume 59, Issue 2, pp 115–121 | Cite as

Lycopene suppresses LPS-induced NO and IL-6 production by inhibiting the activation of ERK, p38MAPK, and NF-κB in macrophages

  • Dan Feng
  • Wen-Hua Ling
  • Rui-Dong Duan
Original Research Paper

Abstract

Background

Lycopene has antioxidant, anticancer, and anti-inflammatory effects with molecular mechanisms not fully identified.

Aim and methods

We investigated the effects of lycopene on the inflammatory responses to lipopolysaccharide (LPS) in RAW264.7 cells and the signal transduction pathways involved.

Results

Lycopene inhibited LPS-induced production of nitric oxide (NO) and interleukin-6 (IL-6) with decreased mRNAs of inducible nitric oxide synthase and IL-6 but had no effect on TNF-α. Further study showed that lycopene also inhibited LPS-induced IκB phosphorylation, IκB degradation, and NF-κB translocation. Moreover, lycopene blocked the phosphorylation of ERK1/2 and p38 MAP kinase but not c-Jun NH2-terminal kinase. To confirm the causal link between MAP kinase inhibition and its anti-inflammatory effects, we treated the cells with SB 203580 and U0126. These inhibitors significantly inhibited LPS-induced NO and IL-6 formation.

Conclusion

Lycopene inhibits the inflammatory response of RAW 264.7 cells to LPS through inhibiting ERK/p38 MAP kinase and the NF-κB pathway.

Keywords

Lycopene Interleukin-6 Nitric oxide p38 MAP kinase ERK1/2 

Notes

Acknowledgments

This work was supported by research grants from the National Basic Research Program (973, No. 2006CB503902), China, the eleventh “five year” national research program of China (2008BAI58B06, 2009–2010), and by a research grant from the Swedish Nutrition Foundation and Lund University Hospital, Lund, Sweden.

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Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  1. 1.Department of Nutrition, School of Public HealthSun Yat-sen University (Northern Campus)GuangzhouPeople’s Republic of China
  2. 2.Gastroenterology and Nutrition Lab, Biomedical Center B11, Institution of Clinical SciencesLund UniversityLundSweden

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