feG-COOH blunts eosinophilic airway inflammation in a feline model of allergic asthma
Objective and design
This study investigated if feG-COOH would decrease allergen-induced airway inflammation.
Materials or subjects
Seven adult cats sensitised to Bermuda grass allergen (BGA) to induce an asthmatic phenotype.
Cats were randomized to receive either feG-COOH (1 mg/kg, PO) or placebo (saline 1 ml, PO) immediately prior to BGA aerosol challenge in a cross-over design.
Bronchoalveolar lavage fluid (BALF) was collected and airway inflammatory response assessed via inflammatory cell number and type; IL-4, IFN-γ and nitric oxide metabolite concentrations. A paired t test was used to compare parameters with a P < 0.05 considered significant.
The BALF eosinophil percentage was significantly lower in asthmatic cats treated with feG compared with placebo (placebo, 35.3 ± 12.2%; feG, 22.4 ± 8.6%; P = 0.002). Treatment with feG did not result in a significant change in any other parameter measured.
These data indicate that a single dose of feG-COOH partially attenuates eosinophilic airway inflammation in experimental feline asthma.
KeywordsCat Immunomodulator Allergy Cytokine Bronchoalveolar lavage
The authors would like to thank Craig Woods and Imulan BioTherapeutics for their generous donation of feG-COOH. Additionally, the authors appreciate the assistance of Rachael Cohen this project.
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