Inflammation Research

, Volume 58, Issue 3, pp 143–150

Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney

  • K. J. Jung
  • E. K. Lee
  • J. Y. Kim
  • Y. Zou
  • B. Sung
  • H. S. Heo
  • M. K. Kim
  • J. Lee
  • N. D. Kim
  • B. Pal Yu
  • H. Y. Chung
Article

DOI: 10.1007/s00011-008-7227-2

Cite this article as:
Jung, K.J., Lee, E.K., Kim, J.Y. et al. Inflamm. Res. (2009) 58: 143. doi:10.1007/s00011-008-7227-2

Abstract.

Objective:

To compare the effect of short-term calorie restriction (CR) on aging with that of already known long-term CR, the anti-inflammatory efficacy of 10-day CR was explored in aged rat kidney.

Treatment:

Two different age groups, 6 months (young) and 24 months (old) were used. In the old group, one sub-group was control, fed ad libitum (AL) and the other was fed CR for 10 days with 40 % of the food intake of the AL subgroup (n = 5).

Methods:

Reactive species (RS), lipid peroxides and COX-2 activity were measured. The activities of proinflammatory transcription factors NF-kB and AP-1 were measured by electro-mobility shift assay (EMSA). Upstream signaling cascades of NF-kB and AP-1 as well as proinflammatory gene expression were detected by Western blot.

Results:

10-day CR suppressed RS, lipid peroxides, and COX-2 activity in aged rat kidney. CR also inhibited upstream signaling cascades and DNA binding activity of NF-kB and AP-1, and thioredoxin/Ref-1 pathway. CR blocked expression of NF-kB-and AP-1-responsive gene COX-2, iNOS, VCAM-1 and ICAM-1.

Conclusions:

We report for the first time that 10-day CR can attenuate the altered signaling transduction for inflammatory processes which is mediated through RS-induced NF-kB and AP-1 in aged kidney.

Keywords:

Aging Short term calorie restriction Molecular inflammation NF-kB AP-1 

Copyright information

© Birkhäuser Verlag, Basel 2009

Authors and Affiliations

  • K. J. Jung
    • 1
  • E. K. Lee
    • 1
  • J. Y. Kim
    • 1
  • Y. Zou
    • 1
  • B. Sung
    • 1
  • H. S. Heo
    • 1
  • M. K. Kim
    • 2
  • J. Lee
    • 1
    • 2
  • N. D. Kim
    • 1
    • 2
  • B. Pal Yu
    • 2
    • 3
  • H. Y. Chung
    • 1
    • 2
  1. 1.Department of Pharmacy, College of PharmacyPusan National UniversityBusanKorea
  2. 2.Longevity Life Science and Technology InstitutesPusan National UniversityBusanKorea
  3. 3.Department of PhysiologyThe University of Texas Health Science CenterSan AntonioUSA