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Inflammation Research

, Volume 58, Issue 3, pp 143–150 | Cite as

Effect of short term calorie restriction on pro-inflammatory NF-kB and AP-1 in aged rat kidney

  • K. J. Jung
  • E. K. Lee
  • J. Y. Kim
  • Y. Zou
  • B. Sung
  • H. S. Heo
  • M. K. Kim
  • J. Lee
  • N. D. Kim
  • B. Pal Yu
  • H. Y. ChungEmail author
Article

Abstract.

Objective:

To compare the effect of short-term calorie restriction (CR) on aging with that of already known long-term CR, the anti-inflammatory efficacy of 10-day CR was explored in aged rat kidney.

Treatment:

Two different age groups, 6 months (young) and 24 months (old) were used. In the old group, one sub-group was control, fed ad libitum (AL) and the other was fed CR for 10 days with 40 % of the food intake of the AL subgroup (n = 5).

Methods:

Reactive species (RS), lipid peroxides and COX-2 activity were measured. The activities of proinflammatory transcription factors NF-kB and AP-1 were measured by electro-mobility shift assay (EMSA). Upstream signaling cascades of NF-kB and AP-1 as well as proinflammatory gene expression were detected by Western blot.

Results:

10-day CR suppressed RS, lipid peroxides, and COX-2 activity in aged rat kidney. CR also inhibited upstream signaling cascades and DNA binding activity of NF-kB and AP-1, and thioredoxin/Ref-1 pathway. CR blocked expression of NF-kB-and AP-1-responsive gene COX-2, iNOS, VCAM-1 and ICAM-1.

Conclusions:

We report for the first time that 10-day CR can attenuate the altered signaling transduction for inflammatory processes which is mediated through RS-induced NF-kB and AP-1 in aged kidney.

Keywords:

Aging Short term calorie restriction Molecular inflammation NF-kB AP-1 

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Copyright information

© Birkhäuser Verlag, Basel 2009

Authors and Affiliations

  • K. J. Jung
    • 1
  • E. K. Lee
    • 1
  • J. Y. Kim
    • 1
  • Y. Zou
    • 1
  • B. Sung
    • 1
  • H. S. Heo
    • 1
  • M. K. Kim
    • 2
  • J. Lee
    • 1
    • 2
  • N. D. Kim
    • 1
    • 2
  • B. Pal Yu
    • 2
    • 3
  • H. Y. Chung
    • 1
    • 2
    Email author
  1. 1.Department of Pharmacy, College of PharmacyPusan National UniversityBusanKorea
  2. 2.Longevity Life Science and Technology InstitutesPusan National UniversityBusanKorea
  3. 3.Department of PhysiologyThe University of Texas Health Science CenterSan AntonioUSA

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