Inflammation Research

, Volume 57, Issue 3, pp 118–125 | Cite as

Immunosuppressive properties of surfactant in alveolar macrophage NR8383

  • J. Kerecman
  • S. B. Mustafa
  • M. M. Vasquez
  • P. S. Dixon
  • R. Castro
Article

Abstract.

Objective:

To evaluate the anti-inflammatory effects of exogenous surfactants and surfactant phospholipid without surfactant proteins (SP-A and SP-D) on the lipopolysaccharide- (LPS) stimulated rat alveolar macrophage (AM) cell line NR8383.

Methods:

Exogenous surfactants (beractant, calfactant or colfosceril) and surfactant phospholipid (dipalmitoyl phosphatidylcholine, DPPC), standardized to phospholipid content of 25–1,000 μg/ml were incubated with LPS- (1 μg/ml) stimulated NR8383 AMs.

Results:

TNF-α and IL-1β secretion and nitric oxide (NO) formation following LPS stimulation were inhibited by treatment with surfactants or DPPC. Furthermore, LPS-dependent NO production and iNOS protein levels were significantly suppressed in cells pretreated for one hour with beractant compared to beractant added simultaneously with or following LPS. Additionally, LPS-stimulated oxidative burst, measured by flow cytometry, was significantly decreased by beractant. Finally, beractant inhibited the translocation of NF-κB from cytoplasmic into nuclear extract in LPS-stimulated NR8383 AMs.

Conclusions:

Exogenous surfactants and surfactant phospholipid inhibit secretion of proinflammatory cytokines and NO in NR8383 AMs. The inhibitory effects of beractant on oxygen radical and LPS-induced NO formation may result from unique mechanisms of decreasing cell signaling. The anti-inflammatory activity of surfactant products used in the treatment of neonatal respiratory distress syndrome (RDS) may depend upon the specific preparation or dose used.

Key words:

Alveolar macrophages Surfactant Nitric oxide (NO) Cytokines LPS 

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Copyright information

© Birkhaueser 2008

Authors and Affiliations

  • J. Kerecman
    • 1
  • S. B. Mustafa
    • 2
  • M. M. Vasquez
    • 2
  • P. S. Dixon
    • 1
  • R. Castro
    • 2
  1. 1.Department of PediatricsWilford Hall USAF Medical CenterSan AntonioUSA
  2. 2.Department of PediatricsUniversity of Texas Health Science Center at San AntonioSan AntonioUSA

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