Inflammation Research

, Volume 56, Issue 9, pp 377–384 | Cite as

Altered protein profile of lymphocytes in an antigen-specific model of colitis: A comparative proteomic study

  • B.-G. Liu
  • Y.-B. Cao
  • Y.-Y. Cao
  • J.-D. Zhang
  • M.-M. An
  • Y. Wang
  • P.-H. Gao
  • L. Yan
  • Y. Xu
  • Y.-Y. Jiang

Abstract.

Objective:

Lymphocytes are deeply involved in the initiation and perpetuation of inflammatory response in inflammatory bowel disease (IBD) and lymphocyte-derived proteins are associated with the pathogenesis of the disease. The aim of this study was to identify the altered protein profiles of lymphocytes from rats with colitis.

Methods:

Colitis models were induced by colonic administration of trinitrobenzene sulfonic acid (TNBS) in 50% ethanol in male SD rats. Seven days after administration of TNBS/ethanol, lymphocytes were harvested from mesenteric lymph nodes (MLNs) and proteins were extracted. Two-dimensional polyacrylamide gel electrophoresis and PDQuest 2D-image-analysis software were used to display and analyze the protein spots. The differentially-expressed proteins were identified by tryptic in-gel digestion and mass spectrometry. Real-time RT-PCR was used for selected transcripts to validate the findings of the proteomics analysis.

Results:

A total of 1,100 protein spots including 26 proteins with at least a two-fold difference in abundance between colitis and control groups were identified. Among all the detected spots, 17 were up-regulated and 9 were down-regulated. It was found that the altered proteins included the regulators of the cell cycle and cell proliferation, signal transduction factors, inflammatory factors, apoptosis-related proteins and metabolic enzymes.

Conclusions:

In lymphocytes of rats with TNBS-induced colitis, 26 altered proteins were identified. They involve inflammation, apoptosis, metabolism, and regulation of the cell cycle, cell proliferation, and signal transduction.

Keywords:

Colitis Inflammatory bowel disease Lymphocyte Proteomics 

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Copyright information

© Birkhäuser Verlag, Basel 2007

Authors and Affiliations

  • B.-G. Liu
    • 1
  • Y.-B. Cao
    • 1
  • Y.-Y. Cao
    • 2
  • J.-D. Zhang
    • 1
  • M.-M. An
    • 1
  • Y. Wang
    • 1
  • P.-H. Gao
    • 1
  • L. Yan
    • 1
  • Y. Xu
    • 1
  • Y.-Y. Jiang
    • 1
  1. 1.Department of Pharmacology, School of PharmacySecond Military Medical UniversityShanghaiChina
  2. 2.Department of Biochemical Pharmacy, School of PharmacySecond Military Medical UniversityShanghaiChina

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