COX-2 mRNA expression decreases in the subplantar muscle of rat paw subjected to carrageenan-induced inflammation after low level laser therapy
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Objective and design:
Low level laser therapy (LLLT) has been presented as a novel therapy to treat inflammation. Herein we studied the effect of LLLT on the COX-2 mRNA expression in subplantar tissue taken from rats treated with carrageenan.
The groups consisted of 32 rats: A1 (Saline), A2 (Carrageenan), A3 (Carrageenan + laser 660 nm) and A4 (Carrageenan + laser 684 nm).
A3 and A4 were irradiated in the first hour after carrageenan.
The edema was measured by a plethysmometer and COX-2 mRNA was by RT-PCR. Statistical were evaluated by ANOVA and Tukey-Kramer Test.
Carrageenan increased both edema (A1= 0.6 ± 0.04 vs. A2= 2.24 ± 0.08) and COX-2 mRNA (A1= 1.1 ± 0.26 vs. A2= 3.52 ± 0.69). Irradiation reduced the edema (A3= 0.84 ± 0.09; A4= 1.31 ± 0.05) and the COX-2 mRNA (A3= 2.16 ± 0.28; A4= 1.86 ± 0.20).
LLLT reduce the expression of COX-2 mRNA.