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Inflammation Research

, Volume 55, Issue 5, pp 200–206 | Cite as

Earlier onset of neutrophil-mediated inflammation in the ultraviolet-exposed skin of mice deficient in myeloperoxidase and NADPH oxidase

  • J. Komatsu
  • H. Koyama
  • N. Maeda
  • Y. Aratani
Original Research Paper

Abstract.

Objective and design

This study examined the role of neutrophil-derived reactive oxygen species (ROS) in neutrophil recruitment into ultraviolet B (UVB)-exposed skin of mice.

Methods

Mouse dorsal skin was irradiated with UVB (600 mJ/cm2). Accumulation of neutrophils within the inflammatory sites was observed histochemically. Keratinocyte-derived chemokine (KC) and macrophage inflammatory protein 2 (MIP-2) were quantified, and in vivo chemotaxis of neutrophils toward KC and MIP-2 was examined.

Results

UVB exposure of mice deficient in myeloperoxidase (MPO), NADPH oxidase, or both, caused skin neutrophil infiltration peaking at 60, 48, and 48 h, respectively, which was earlier than the 72-h peak in wild-type mice. MIP-2 level was higher in mutant than wild-type mice. Mutant neutrophils produced more MIP-2 in vitro. Neutrophil migration toward a localized source of KC was higher in mutant than wild type mice. NADPH oxidase deficiency had a greater effect on migration than MPO deficiency.

Conclusions

These results suggest that ROS produced by neutrophils regulate expression of MIP-2 and migration of neutrophils toward KC. This may explain the earlier infiltration of mutant neutrophils in response to UVB.

Keywords.

Inflammation Myeloperoxidase NADPH oxidase Neutrophil Ultraviolet 

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Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  1. 1.Kihara Institute for Biological ResearchYokohama City UniversityYokohamaJapan
  2. 2.Department of Pathology and Laboratory MedicineUniversity of North CarolinaChapel HillUSA

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