Deficiency of regulatory B cells increases allergic airway inflammation
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Objective: To investigate the effect of the X-linked immunodeficiency (Xid) B cell defect on the response to the cockroach allergen in mice.
Methods: Two cockroach allergen immunization and challenge protocols were employed to sensitize CBA/J wild-type and CBA/CaHN-btk(-/-)xid/J (Xid) mice. Blood and tissue samples were collected 24 and 48 hrs after the last intratracheal antigen challenge and were analyzed for several parameters of allergic inflammation.
Results: Nearly equivalent amounts of serum IgE were detected in Xid and CBA/J mice after short-term antigen challenge despite the B cell deficiency in Xid mice. A decreased concentration of IgE was detected in CBA/J mice after repeated allergen challenges but not in the Xid mice. Correlating with the discrepancy in serum IgE levels, higher levels of IL-13, IL-5, IL-10 and CCL5 were measured in whole lung homogenates from allergen-challenged Xid mice compared to CBA/J mice. In addition, draining lymph node cells from Xid mice expressed elevated levels of IL-4, IL-5, IL-10 and IFNγ mRNA compared to cells from CBA/J mice after in vitro culture with cockroach antigen. An increase in lung inflammation, interstitial eosinophilia and mucus production was also observed in allergen-challenged Xid mice. CD95L expression increased on B-1a cells following allergen challenge, which was accompanied by an increase in lung CD4+ Th cell apoptosis in wild-type CBA/J mice. In contrast, Xid mice did not have an increase in CD4+ T cell apoptosis following allergen challenge.
Conclusions: These data suggest a regulatory role for B-1a cells in reducing cytokine production, pulmonary inflammation, and CD4+ T cell survival during cockroach allergen-induced airway inflammation.
Key words.Allergy B cells T cells Inflammation Apoptosis
- B-1a cells
CD5+ B cells
CD95 ligand or Fas ligand
- Btk -/-
Bruton’s tyrosine kinase deficient
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