Inflammation Research

, Volume 54, Issue 1, pp 17–21

Anti-inflammatory activity of PAS-1, a protein component of Ascaris suum

  • T. M. Oshiro
  • M. S. Macedo
  • M. F. Macedo-Soares
Original Research Paper

DOI: 10.1007/s00011-004-1316-7

Cite this article as:
Oshiro, T.M., Macedo, M.S. & Macedo-Soares, M.F. Inflamm. res. (2005) 54: 17. doi:10.1007/s00011-004-1316-7

Abstract.

Background: Recently, we identified a 200 kDa protein (PAS-1) from Ascaris suum worms, that suppresses the humoral immune response. Here, the effect of PAS-1 on inflammatory leukocyte migration induced by bacterial lipopolysaccharide (LPS) was investigated.

Methods: Cellular migration and cytokine release, stimulated by LPS or LPS+PAS-1, were analyzed in air pouches induced in the shaved back of BALB/c mice. Cytokines were determined by ELISA and RT-PCR on air pouch exudates and in vitro stimulated peritoneal macrophages.

Results: The significant cellular influx induced by LPS, consisting predominantly of neutrophils, was highly suppressed in the presence of PAS-1, but not a non-related protein. PAS-1 led also to a marked reduction of TNF-α, IL-1 β and IL-6 levels in both LPS-stimulated air pouches and peritoneal macrophage cultures. In contrast, PAS-1 induced a significant increase of IL-10 and TGF-β production.

Conclusions: These results demonstrate that PAS-1 has a potent anti-inflammatory activity, probably due to the stimulation of regulatory cytokines in macrophages, thus leading to the inhibition of pro-inflammatory cytokine production.

Key words.

Ascaris suum Immunomodulation Inflammation Pro-inflammatory cytokine Regulatory cytokine 

Copyright information

© Birkhäuser Verlag, Basel 2005

Authors and Affiliations

  • T. M. Oshiro
    • 1
  • M. S. Macedo
    • 2
  • M. F. Macedo-Soares
    • 1
  1. 1.Laboratory of ImmunopathologyButantan InstituteSão PauloBrasil
  2. 2.Department of Immunology, Institute of Biomedical SciencesUniversity of São PauloSão PauloBrazil

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