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Inflammation Research

, Volume 53, Issue 12, pp 658–663 | Cite as

Evidence that arachidonic acid derived from neutrophils and prostaglandin E2 are associated with the induction of acute lung inflammation by lipopolysaccharide of Escherichia coli

  • T. C. Alba-LoureiroEmail author
  • E. F. Martins
  • C. K. Miyasaka
  • L. R. Lopes
  • R. G. Landgraf
  • S. Jancar
  • R. Curi
  • P. Sannomiya
Original Research Paper

Abstract.

Objective: The involvement of arachidonic acid (AA) and PGE2 during the E. coli lipopolysaccharide (LPS)-induced acute lung injury was investigated.

Material: Adult male Wistar rats were used. For in vitro studies, rat neutrophils, bronchoalveolar lavage (BAL) fluid, and lug vascular endothelium were used, as described below.

Treatment: Rats were given an intratracheal injection of LPS (750 μg).

Methods: Total and differential cell counts in BAL fluid; enzyme-linked immunoassay (ELISA) analyses of TNF-α, IL-1β, LTB4 and PGE2 in BAL, and immunohistochemical detection of ICAM-1 on lung vascular endothelium were performed six h after LPS challenge. Fatty acid composition of blood neutrophils and plasma was analyzed by HPLC.

Results: Rats instilled with LPS presented a sixty three-fold increase in the number of neutrophils in BAL (from 0.5 × 106 to 31.5 × 106 cells), accompanied by increased levels of TNF-α and IL-1β (p < 0.001), and a three-fold increase in ICAM-1 expression on vascular endothelium. The content of AA in blood neutrophils was reduced by 50%, whereas the level of PGE2 in BAL was increased by 3.5 fold, without changes in the levels of LTB4.

Conclusions: These findings suggest that AA and PGE2 are associated with LPS challenge.

Key words.

Neutrophils Lipopolysaccharide Acute lung injury PGE2 Arachidonic acid 

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Copyright information

© Birkhäuser Verlag, Basel 2004

Authors and Affiliations

  • T. C. Alba-Loureiro
    • 1
    Email author
  • E. F. Martins
    • 2
  • C. K. Miyasaka
    • 2
  • L. R. Lopes
    • 1
  • R. G. Landgraf
    • 3
  • S. Jancar
    • 3
  • R. Curi
    • 2
  • P. Sannomiya
    • 4
  1. 1.Department of Pharmacology, Institute of Biomedical SciencesUniversity of São PauloSão PauloBrazil
  2. 2.Department of Physiology and Biophysics, Institute of Biomedical SciencesUniversity of São PauloBrazil
  3. 3.Department of Immunology, Institute of Biomedical SciencesUniversity of São PauloBrazil
  4. 4.Research Division, Institute of Heart (INCOR), LIM-11University of São Paulo Medical SchoolSão PauloBrazil

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