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Inflammation Research

, Volume 53, Issue 9, pp 484–488 | Cite as

Single nucleotide polymorphisms and haplotypes of histamine N-methyltransferase in patients with gastric ulcer

  • G. -L. Chen
  • B. Zhu
  • W. -P. Nie
  • Z. -H. Xu
  • Z. -R. Tan
  • G. Zhou
  • J. Liu
  • W. Wang
  • H. -H. Zhou
Original Paper

Abstract.

Introduction: Histamine plays a crucial role in the regulation of gastric acid secretion, which is involved in the pathogenesis of peptic ulcer. Histamine N-methyltransferase (HNMT) is the major metabolizing enzyme for histamine inactivation in human stomach.

Objective: This study aims to determine whether there exists a relationship between HNMT gene polymorphisms and the risk for gastric ulcer (GU).

Methods: 118 GU patients and 154 ethnically matched control subjects were enrolled and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were developed to genotype all these subjects for the T-1637C, C-411T, C314T and A1097T point mutations in HNMT gene. Haplotypes were reconstructed from the genotype data.

Results: Frequencies of the variant alleles in cases and controls were 0.398 vs 0.396 for T-1637C, 0.144 vs 0.110 for C-411T, 0.034 vs 0.042 for C314T, and 0.242 vs 0.273 for A1097T, respectively, with no significant difference for any locus between the two groups (all P > 0.05). Also the frequencies of genotypes, haplotypes and haplotype pairs based on these polymorphisms did not differ significantly between cases and controls.

Conclusion: This study provided no evidence for the involvement of HNMT polymorphisms in the susceptibility to GU.

Gastric ulcer Histamine N-methyltransferase Single nucleotide polymorphism Haplotype Case-control study 

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Copyright information

© Birkhäuser Verlag, Basel 2004

Authors and Affiliations

  • G. -L. Chen
    • 1
  • B. Zhu
    • 1
  • W. -P. Nie
    • 2
  • Z. -H. Xu
    • 3
  • Z. -R. Tan
    • 1
  • G. Zhou
    • 1
  • J. Liu
    • 1
  • W. Wang
    • 4
  • H. -H. Zhou
    • 1
  1. 1.Pharmacogenetics Research Institute, Institute of Clinical PharmacologyCentral South UniversityChangsha, HunanP.R. China
  2. 2.3rd Xiang-Ya Hospital of Central South UniversityChangsha, HunanP.R. China
  3. 3.Clinical Drug EvaluationJohnson & Johnson Pharmacoceutical Research & DevelopmentUSA
  4. 4.Department of Pathology, HEBMedical College of OhioToledoUSA

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