Inflammation Research

, Volume 53, Issue 6, pp 253–256

An immunoglobulin agent (IVIG) inhibits NF-κB activation in cultured endothelial cells of coronary arteries in vitro

  • T. Ichiyama
  • Y. Ueno
  • H. Isumi
  • A. Niimi
  • T. Matsubara
  • S. Furukawa
Original Paper

Abstract

Objective:Kawasaki disease (KD) is an acute febrile vasculitis of unknown etiology that may lead to cardiovascular disorders. High-dose intravenous immunoglobulin (IVIG) therapy is well established as a standard therapy for KD. Tumor necrosis factor-α (TNF-α) is responsible for the pathogenesis of acute KD. We examined whether or not IVIG inhibits TNF-α-induced activation of transcription factor NF-κB, a factor that is essential for the expression of proinflammatory cytokines, in human coronary artery endothelial cells (CAEC).

Methods:The inhibitory effect of IVIG on NF-κB activation induced by TNF-α was evaluated by Western blot analysis and ELISA. Moreover, the inhibitory effects of IVIG on IκBα degradation, interleukin-6 (IL-6) production, and E-selectin expression induced by TNF-α were evaluated by Western blot analysis, ELISA, and flow cytometry, respectively.

Results:Western blot analysis and ELISA demonstrated that IVIG inhibits NF-κB activation induced by TNF-α in CAEC. Moreover, IVIG inhibited IκBα degradation, IL-6 production, and E-selectin expression induced by TNF-α in CAEC.

Conclusions:The data suggest that IVIG inhibits NF-κB activation induced by TNF-α in CAEC, thereby possibly modulating IL-6 production and E-selectin expression.

Coronary artery endothelial cells E-Selectin Interleukin-6 Intravenous immunoglobulin NF-κB 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Birkhäuser-Verlag Basel 2004

Authors and Affiliations

  • T. Ichiyama
    • 1
  • Y. Ueno
    • 1
  • H. Isumi
    • 1
  • A. Niimi
    • 2
  • T. Matsubara
    • 1
  • S. Furukawa
    • 1
  1. 1.Department of PediatricsYamaguchi University School of MedicineYamaguchiJapan
  2. 2.Bayer Yakuhin Ltd.OsakaJapan

Personalised recommendations