The migration of bone marrow-derived non-hematopoietic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner

  • Magda Kucia
  • Wojtek Wojakowski
  • Ryan Reca
  • Bogdan Machalinski
  • Jolanta Gozdzik
  • Marcin Majka
  • Jarek Baran
  • Janina Ratajczak
  • Mariusz Z. Ratajczak
Original Article

Abstract.

Introduction: Recently we identified in bone marrow (BM) by employing chemotactic isolation to SDF-1 gradient combined with real time RT-PCR analysis a mobile population of CXCR4+ BM mononuclear cells that express mRNA for various markers of early tissue-committed stem cells (TCSCs). In this study we evaluated whether TCSCs respond to other motomorphogens, such as hepatocyte growth factor (HGF) and leukemia inhibitory factor (LIF).

Materials and Methods: We again employed chemotactic isolation combined with real-time RT-PCR analysis to assess whether murine and human BM contain TCSCs that respond to HGF and LIF gradients. We also evaluated expressions of HGF and LIF in damaged organs.

Results: We noted that the number of TCSCs is highest in BM from young (1- to 2-month-old) mice and decreases in 1-year–old animals. Murine and human TCSCs 1) respond to HGF and LIF gradients in addition to an SDF-1 gradient, 2) reside in populations of BM-derived non-hematopoietic CD45cells, and 3) are released (mobilized) from BM into the peripheral blood (PB) during tissue injury (e.g. after partial body irradiation).

Conclusions: These findings further support our theory of the BM as a “hideout” for TCSCs and we suggest that their presence in BM tissue should be considered before experimental evidence is interpreted simply as transdifferentiation/plasticity of hematopoietic stem cells. Since we demonstrated that not only SDF-1, but also HGF and LIF are upregulated in damaged tissues, we postulate that CXCR4+ c-Met+ LIF-R+ TCSC could be mobilized from the BM into the PB, from which they are subsequently chemoattracted to damaged organs, where they play a role in tissue repair/regeneration.

Keywords.

CXCR4-SDF-1 c-MET-HGF LIF-R-LIF stem cell plasticity stem cell mobilization regeneration 

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Copyright information

© Birkhäuser Verlag, Basel 2006

Authors and Affiliations

  • Magda Kucia
    • 1
  • Wojtek Wojakowski
    • 2
  • Ryan Reca
    • 1
  • Bogdan Machalinski
    • 3
  • Jolanta Gozdzik
    • 4
  • Marcin Majka
    • 4
  • Jarek Baran
    • 4
  • Janina Ratajczak
    • 1
  • Mariusz Z. Ratajczak
    • 1
    • 4
  1. 1.Stem Cell Biology Program at the James Graham Brown Cancer CenterUniversity of LouisvilleLouisvilleUSA
  2. 2.Third Division of CardiologySilesian School of MedicineKatowicePoland
  3. 3.Pomeranian Medical School of MedicineSzczecinPoland
  4. 4.European Stem Cell Therapeutic Excellence Center,Medical CollegeJagiellonian UniversityCracowPoland

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