Biofilm-formation by drug-resistant Staphylococcus aureus from cow milk

  • Renata Albuquerque CostaEmail author
  • Jeniffer Vasconcelos de Lira
  • Márcia Facundo Aragão
Research Article


The present study aims to quantify biofilm formation and determine the drug resistance profile of Staphylococcus aureus isolated from raw, pasteurized and ultra-high temperature (UHT) milk. Detection of S. aureus was made in 90 cow milk samples (30 raw, 30 pasteurized and 30 UHT). Biofilm biomass quantification was performed in 96-well polystyrene plates using the crystal violet technique. The resistance profile was determined by the disc-diffusion test. S. aureus was detected in 24.4% (22/90) of milk samples, with the highest prevalence in pasteurized milk. Biofilm production was observed in 100% of the S. aureus isolates from pasteurized and UHT milk, and in 81.8% of the strains from raw milk. Biofilm production by S. aureus drug-resistant strains was detected in 46.7% (14/30) of the isolates. In order to mitigate this problem, it is recommended to apply appropriate hygiene practices along the milk production chain.


Bovine milk Staphylococci Bacterial biofilm Antibiotic resistance 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL) 2018

Authors and Affiliations

  • Renata Albuquerque Costa
    • 1
    Email author
  • Jeniffer Vasconcelos de Lira
    • 1
  • Márcia Facundo Aragão
    • 1
  1. 1.INTA University Center - UNINTASobralBrazil

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