Pharmacognostic standardization and antioxidant capacity of an edible mushroom Laetiporus sulphureus

  • Krishnendu Acharya
  • Sandipta Ghosh
  • Somanjana Khatua
  • Payel Mitra
Research Article


Traditionally Laetiporus sulphureus, commonly known as “Chicken-of-the-Woods”, is considered a delicacy in various parts of the world and recently has shown medical potential in several treatments such as antitumor, antimicrobial, anti-inflammatory, antioxidant, immune-modulating etc. However, quality standards of this species have not been studied so far. For this purpose, microscopic features of powder such as physical characters, spore measurement, type of hyphae were examined and recorded. Physicochemical parameters like organoleptic features, loss on drying (5.15 %), total ash (3.04 %), swelling capacity (1.66 ml/g), water holding capacity (163.16 g/g), water solubility index (21.73 %) and fluorescent behaviour against 16 reagents were also determined. In addition, alcoholic extract was prepared using methanol as solvent where extractive value was 24.27 %. Preliminary mycochemical analysis indicated presence of cardiac glycoside, flavonoid, phenol, saponin and terpenoids in the extract. While quantitatively major bioactive components were present in the following order of flavonoid > phenol > ascorbic acid > β-carotene > lycopene. Moreover, HPTLC and HPLC profiles were recorded to determine phenolic fingerprint. HPTLC characterization showed presence of at least 5 components as detected by scanning the plate at 300 nm and HPLC chromatogram indicated existence of 32 peaks which might be of phenolic compounds. In addition, superoxide radical scavenging (EC50 1.38 mg/ml), DPPH radical scavenging (EC50 0.11 mg/ml), chelating ability of ferrous ion (EC50 0.27 mg/ml), reducing power (EC50 0.26 mg/ml) and total antioxidant capacity (18.01 µg AAE/mg of extract) were determined to evaluate antioxidant potentiality of the extract. The above parameters are significant towards establishing pharmacognostic standards for future authentication of genuine mushroom material.


Antioxidant property HPLC HPTLC Medicinal edible mushroom Pharmacognostic standardization Physico-chemical evaluation 


  1. Bhattacharya S, Zaman MK (2009) Pharmacognostical evaluation of Zanthoxylum nitidum bark. Int J Pharm Tech Res 1(2):292–298Google Scholar
  2. Biswas G, Acharya K (2013) Hypoglycemic activity of ethanolic extract of Astraeus hygrometricus (Pers.) Morg. in alloxan-induced diabetic mice. Int J Pharm Pharm Sci 5(1):391–394Google Scholar
  3. Biswas G, Sarkar S, Acharya K (2011) Hepatoprotective activity of the ethanolic extract of Astraeus hygrometricus (Pers.) Morg. Dig J Nanomater Bios 6:637–641Google Scholar
  4. Chatterjee S, Saha GK, Acharya K (2011) Antioxidant activities of extracts obtained by different fractionation from Tricholoma giganteum basidiocarps. Pharmacologyonline 3:88–97Google Scholar
  5. Chatterjee A, Khatua S, Chatterjee S, Paloi S, Mukherjee S, Mukherjee A, Acharya K, Bandyopadhyay SK (2013) Polysaccharide-rich fraction of Termitomyces eurhizus accelerate healing of indomethacin induced gastric ulcer in mice. Glycoconj J 30:759–768CrossRefPubMedGoogle Scholar
  6. Chen H, Ju Y, Li J, Yu M (2012) Antioxidant activities of polysaccharides from Lentinus edodes and their significance for disease prevention. Int J Bio Macromol 50:214–218CrossRefGoogle Scholar
  7. Dash S, Nath LK, Bhise S, Bhuyan N (2005) Antioxidant and antimicrobial activities of Heracleum nepalense D Don root. Trop J Pharm Res 4(1):341–347Google Scholar
  8. Dinis TCP, Mudaira VMC, Alnicida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-amino salicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169CrossRefPubMedGoogle Scholar
  9. Dutta AK, Acharya K (2014) Traditional and ethno-medicinal knowledge of mushrooms in West Bengal, India. Asian J Pharm Clin Res 7(4):36–41Google Scholar
  10. Ericsson DCB, Ivonne JNR (2009) Sterol composition of the macromycete fungus Laetiporus sulphureus. Chem Nat Compd 45(2):193–196CrossRefGoogle Scholar
  11. Ferreira ICFR, Baptista P, Vilas-Boas M, Barros L (2007) Free-radical scavenging capacity and reducing power of wild edible mushrooms from northeast Portugal: individual cap and stipe activity. Food Chem 100:1511–1516CrossRefGoogle Scholar
  12. Harbone JB (1973) Phytochemical methods. Chapman and Hall Ltd., LondonGoogle Scholar
  13. Indian Pharmacopoeia Commission (2007) Indian pharmacopoeia, vol I. Government of India, New DelhiGoogle Scholar
  14. Khatua S, Paul S, Acharya K (2013) Mushroom as the potential source of new generation of antioxidant: a review. Res J Pharm Technol 6(5):496–505Google Scholar
  15. Khatua S, Dutta AK, Acharya K (2015) Russula senecis: a delicacy among the tribes of West Bengal. PeerJ 3:e810. doi: 10.7717/peerj.810 PubMedCentralCrossRefPubMedGoogle Scholar
  16. Luangharn T, Hyde KD, Chukeatirote E (2014) Proximate analysis and mineral content of Laetiporus sulphureus strain MFLUCC 12-0546 from Northern Thailand. Chiang Mai J Sci 41(4):765–770Google Scholar
  17. Lung M-Y, Chang Y-C (2011) Antioxidant properties of the edible basidiomycete Armillaria mellea in submerged cultures. Int J Mol Sci 12:6367–6384PubMedCentralCrossRefPubMedGoogle Scholar
  18. Mallick S, Dutta A, Dey S, Ghosh J, Mukherjee D, Sultana SS, Mandal S, Paloi S, Khatua S, Acharya K, Pal C (2014) Selective inhibition of Leishmania donovani by active extracts of wild mushrooms used by the tribal population of India: an in vitro exploration for new leads against parasitic protozoans. Exp Parasitol 138:9–17CrossRefPubMedGoogle Scholar
  19. Martinez AC, Marcelo EL, Marco AO, Moacyr M (2001) Differential responses of superoxide dismutase in freezing resistant Solanum curtibolum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress. Plant Sci 160:505–515CrossRefPubMedGoogle Scholar
  20. Mau J-L, Chao G-R, Wu K-T (2001) Antioxidant properties of methanolic extracts from several ear mushrooms. J Agric Food Chem 49:5461–5467CrossRefPubMedGoogle Scholar
  21. Mitra P, Mandal NC, Acharya K (2014) Phytochemical characteristics and free radical scavenging activity of ethanolic extract of Termitomyces microcarpus R Heim. Der Pharmacia Lettre 6(5):92–98Google Scholar
  22. Nandi AK, Sen IK, Samanta S, Maity K, Devi KSP, Mukherjee S, Maiti TK, Achrya K, Islam SS (2012) Glucan from hot aqueous extract of an ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr.: structural characterization and study of immunoenhancing properties. Carbohydr Res 363:43–50CrossRefPubMedGoogle Scholar
  23. Nandi AK, Samanta S, Sen Ipsita K, Sanjana K, Devi P, Maiti Tapas K, Acharya Krishnendu, Islam SS (2013) Structural elucidation of an immunoenhancing heteroglycan isolated from Russula albonigra (Krombh.) Fr. Carbohydr Polym 94:918–926CrossRefPubMedGoogle Scholar
  24. Oyaizu M (1986) Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44:307–315CrossRefGoogle Scholar
  25. Pal J, Ganguly S, Tahsin KS, Acharya K (2010) In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer. Indian J Exp Biol 48:1210–1218PubMedGoogle Scholar
  26. Patra P, Bhanja SK, Sen IK, Nandi AK, Samanta S, Das D, Devi KSP, Maiti TK, Achrya K, Islam SS (2012) Structural and immunological studies of hetero polysaccharide isolated from the alkaline extract of Tricholoma crassum (Bark.) Sacc. Carbohydr Res 362:1–7CrossRefPubMedGoogle Scholar
  27. Patra S, Patra P, Maity KK, Mandal S, Bhunia SK, Dey B, Devi KSP, Khatua S, Acharya K, Maiti TK, Islam SS (2013) A heteroglycan from the mycelia of Pleurotus ostreatus: structure determination and study of antioxidant properties. Carbohydr Res 368:16–21CrossRefPubMedGoogle Scholar
  28. Pattanayak M, Samanta S, Maity P, Sen IK, Nandi AK, Manna DK, Mitra P, Acharya K, Islam SS (2015) Heteroglycan of an edible mushroom Termitomyces clypeatus: structure elucidation and antioxidant properties. Carbohydr Res 413:30–36CrossRefPubMedGoogle Scholar
  29. Petrović J, Papandreou M, Glamočlija J, Ćirić A, Baskakis C, Proestos C, Lamari F, Zoumpoulakis P, Soković M (2014) Different extraction methodologies and their influence on the bioactivity of the wild edible mushroom Laetiporus sulphureus (Bull.) Murrill. Food Funct 5(11):2948–2960. doi: 10.1039/c4fo00727a CrossRefPubMedGoogle Scholar
  30. Pradhan P, Dutta AK, Acharya K (2015) A low cost long term preservation of macromycetes for fungarium. Protoc Exch. doi: 10.1038/protex.2015.026 Google Scholar
  31. Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:334–337CrossRefGoogle Scholar
  32. Rai M, Sen S, Acharya K (2013) Antimicrobial activity of four wild edible mushrooms from Darjeeling Hills, West Bengal India. Int J PharmTech Res 5(3):949–956Google Scholar
  33. Ramalingam N, Ramakrishnan K, Krishnan C, Sankar S (2013) Phytochemical analysis, HPTLC finger printing, in vitro antioxidant and cytotoxic activity of Cymodocea serrulata. Pharmacogn J 5:238–241CrossRefGoogle Scholar
  34. Saha S, Khatua S, Paloi S, Acharya K (2013) Antioxidant and nitric oxide synthase activation properties of water soluble polysaccharides from Pleurotus florida. Int J Green Pharm 7:182–188CrossRefGoogle Scholar
  35. Samanta S, Maity K, Nandi AK, Sen IK, Devi KSP, Mukherjee S, Maiti TK, Acharya K, Islam SS (2013) A glucan from an ectomycorrhizal edible mushroom Tricholoma crassum (Berk.) Sacc.: isolation, characterization and biological studies. Carbohydr Res 367:33–40CrossRefPubMedGoogle Scholar
  36. Sarker SD (2012) Pharmacognosy in modern pharmacy curricula. Pharmacogn Mag 8(30):91–92PubMedCentralCrossRefPubMedGoogle Scholar
  37. Sharma JR (2012) Aphyllophorales of himalaya. Botanical Survey of India, Salt LakeGoogle Scholar
  38. Shimada K, Fujikawa K, Yahara K, Nakamura T (1992) Antioxidative properties of Xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 40:945–948CrossRefGoogle Scholar
  39. Singh R, Singh AP, Dhingra GS, Shri R (2014) Taxonomy, physicochemical evaluation and chemical investigation of Ganoderma applanatum and G. brownie. Int J Adv Res 2(5):702–711Google Scholar
  40. Sonibare MA, Olatubosun OV (2015) Pharmacognostic and free radical scavenging evaluation of Cyathula prostata (Blume) L. Phrmacogn J 7(2):107–116CrossRefGoogle Scholar
  41. Zeković Z, Vidović S, Mujić I (2010) Selenium and Zinc content and radical scavenging capacity of edible mushrooms Armilaria mellea and Lycoperdon saccatum. Croat J Food Sci Technol 2:16–21Google Scholar

Copyright information

© Bundesamt für Verbraucherschutz und Lebensmittelsicherheit (BVL) 2015

Authors and Affiliations

  • Krishnendu Acharya
    • 1
  • Sandipta Ghosh
    • 1
  • Somanjana Khatua
    • 1
  • Payel Mitra
    • 1
  1. 1.Molecular and Applied Mycology and Plant Pathology Laboratory, Department of BotanyUniversity of CalcuttaKolkataIndia

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