Biologia Plantarum

, Volume 49, Issue 1, pp 81–84 | Cite as

Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity

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Abstract

The responses of antioxidative system of rice to chilling were investigated in a tolerant cultivar, Xiangnuo-1, and a susceptible cultivar, IR-50. The electrolyte leakage and malondialdehyde content of Xiangnuo-1 were little affected by chilling treatment but those of IR-50 increased. Activities of suoperoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase, and ascorbic acid content of Xiangnuo-1 were remained high, while those of IR-50 decreased under chilling. The results indicated that higher activities of defense enzymes and higher content of antioxidant under stress were associated with tolerance to chilling.

Additional key words

ascorbate peroxidase ascorbic acid catalase glutathione reductase superoxide dismutase 

Abbreviations

ROS

reactive oxygen species

AsA

ascorbic acid

APX

ascorbate peroxidase

CAT

catalase

GR

glutathione reductase

GSH

glutathione, reduced form

MDA

malondialdehyde

SOD

superoxidase

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References

  1. Bowler, C., Van Montagu, M., Inze, D.: Superoxide dismutase and stress tolerance.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 83–116, 1992.CrossRefGoogle Scholar
  2. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase.-J. exp. Bot. 32: 93–101, 1981.Google Scholar
  3. Fadzillah, N.M., Gill, V., Finch, B.P., Burdon, R.H.: Chilling, oxidative stress and antioxidant responses in shoot cultures of rice.-Planta 199: 552–556, 1996.CrossRefGoogle Scholar
  4. Gamble, P.E., Burke, J.J.: Effect of water stress on the chloroplastic antioxidant system. I. Alterations in glutathione reductase activity.-Plant Physiol. 76: 615–621, 1984.CrossRefPubMedGoogle Scholar
  5. Hodges, D.M., Andrews, C.J., Johnson, D.A., Hamilton, R.I.: Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines.-J. exp. Bot. 48: 1105–1113, 1997a.Google Scholar
  6. Hodges, D.M., Andrews, C.J., Johnson, D.A., Hamilton, R.I.: Antioxidant enzyme and compound responses to chilling stress and their combining abilities in differentially sensitive maize hybrids.-Crop Sci. 37: 857–863, 1997b.CrossRefGoogle Scholar
  7. Kang, H.-M., Saltveit, M.E.: Reduced chilling tolerance in elongating cucumber seedling radicles is related to their reduced antioxidant enzyme and DPPH-radical scavenging activity.-Physiol. Plant. 115: 244–250, 2002.CrossRefPubMedGoogle Scholar
  8. Law, M.Y., Charles, S.A., Halliwell, B.: Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts.-Biochem. J. 210: 899–903, 1983.PubMedGoogle Scholar
  9. Liu, C.-L., Chen, H.-P., Liu, E.-E., Peng, X.-X., Lu, S.-Y., Guo, Z.-F.: Multiple tolerance of rice to abiotic stresses and its relationship with ABA accumulation.-Acta agron. sin. 29: 725–729, 2003.Google Scholar
  10. Lu, S., Guo, Z., Peng, X.: Effects of ABA and S-3307 on drought resistance and antioxidative enzyme activity of turfgrass.-J. Hort. Sci. Biotech. 78: 663–666, 2003.Google Scholar
  11. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxdase in spinach chloroplasts.-Plant Cell Physiol. 22: 867–880, 1981.Google Scholar
  12. O’Kane D., Gill, V., Boyd, P.L, Burdon, R.: Chilling, oxidative stress and antioxidant reponses in Arabidopsis thaliana callus.-Planta 198: 371–377, 1996.CrossRefPubMedGoogle Scholar
  13. Payton, P., Webb, R., Kornyeyev, D., Allen, R., Holadys, A.S.: Protecting cotton photosynthesis during moderate chilling at high light intensity by increasing chloroplastic antioxidant enzyme activity.-J. exp. Bot. 52: 2345–2354, 2001.CrossRefPubMedGoogle Scholar
  14. Prasad, T.K., Anderson, M.D., Martin, B.A., Stewart, C.R.: Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide.-Plant Cell 6: 65–74, 1994.CrossRefPubMedGoogle Scholar
  15. Queiroz, C.G.S., Alonso, A., Mares-Guia, M., Magalhaes, A.C.: Chilling-induced changes in membrane fluidity and antioxidant enzyme activities in Coffea arabica L. roots.-Biol. Plant. 41: 403–413, 1998.CrossRefGoogle Scholar
  16. Sato, Y., Murakami, T., Funatsuki, H., Matsuba, S., Saruyama, H., Tanida, M.: Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings.-J. exp. Bot. 52: 145–151, 2001.CrossRefPubMedGoogle Scholar
  17. Singh, D.V., Srivastava, G.C., Abdin, M.Z.: Amelioration of negative effect of water stress in Cassia angustifolia by benzyladenine and/or ascorbic acid.-Biol. Plant. 41: 141–143, 2001.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany 2005

Authors and Affiliations

  1. 1.College of Life ScienceSouth China Agricultural UniversityGuangzhouChina

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