American Potato Journal

, Volume 49, Issue 6, pp 227–233 | Cite as

Protein comparisons in selected Phureja-haploid Tuberosum families

  • Sharon Desborough
  • C. J. Weiser


Quantitative measurements were made of soluble and total proteins in a study of tuber protein inheritance in selected Phureja-haploid Tuberosum families. The effects of ploidy and location were also examined. A preliminary study comparing methods for measuring tuber proteins revealed that the technique described by Gusta et al., (1), was most suitable for total protein; while the TCA technique was satisfactory for analyzing soluble proteins. The method described by Potty (2) was used in both classes for final protein determination. Both protein classes appeared to be directly influenced by ploidy level and growing location. More important, the relative amounts of tuber protein were substantially increased in the first generation of selection.


Ploidy Level AMERICAN Potato Journal Rockefeller Foundation Percent Protein Tuber Protein 
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Literature Cited

  1. 1.
    Gusta, L. V., D. R. Evert and C. J. Weiser. 1970. A simple method for the extraction and precipitation of total protein and nucleic acids from plant tissues. (In manuscript).Google Scholar
  2. 2.
    Potty, V. H. 1969. Determination of protein in the presence of phenols and pectins. Analyt. Biochem. 537–39.Google Scholar

Copyright information

© Springer 1972

Authors and Affiliations

  • Sharon Desborough
    • 1
  • C. J. Weiser
    • 1
  1. 1.Laboratory of Plant HardinessUniversity of Minnesota, Department of Horticultural ScienceSt. Paul

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