An inoculum technique for the production of fungal pellets
A simple standard inoculation procedure has been developed to obtain growth of fungi in the form of pellets. This technique made use of filamentous mycelium from a preculture as an inoculum, yielding many small pellets with a fairly homogeneous size distribution. At an early stage of growth the presence of a polymer (Carbopol-934) proved to be very important for the way spores germinate and lowered the agglomeration tendency. At a later stage of growth the influence of shearing forces becomes more predominant.
KeywordsPolymer Germinate Agglomeration Homogeneous Size Inoculation Procedure
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- Calam CT (1976) Process biochemistry, April 7–12Google Scholar
- Cimerman A, Johanides V, Skafar S (1976) Paper presented at the Fifth International Fermentation Symposium, BerlinGoogle Scholar
- Elmayergi H (1975) J Ferment Technol 53:722–729Google Scholar
- Karube I, Hirano KI, Suzuki S (1977) Biotechnol Bioeng 19:1233–1238Google Scholar
- Metz B (1976) Ph D Thesis, Delft University of TechnologyGoogle Scholar
- Metz B, Kossen NWF (1977) Biotechnol Bioeng 19:781–799Google Scholar
- Meyrath J, Suchanek J (1972) Methods in microbiology. 7B:159–209Google Scholar
- Morikawa Y, Karube I, Suzuki S (1979) Biotechnol Bioeng 21:261–270Google Scholar
- Suijdam JC van, Kossen NWF, Joha AC (1978) Biotechnol Bioeng 20:1695–1709Google Scholar
- Suzuki S, Karube I (1979) Production of antibiotics and enzymes by immobilized whole cells. ACS Symposium Series 106:59–72Google Scholar
- Tosoni AL, Glass DG (1963) Canadian patent 671.647Google Scholar
- Whitaker A, Long PA (1973) Process biochemistry, November 27–31Google Scholar