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Diabetologia

, Volume 23, Issue 1, pp 1–5 | Cite as

The glycolytic cascade in pancreatic islets

  • W. J. Malaisse
  • F. Malaisse-Lagae
  • A. Sener
Review Articles
  • 36 Downloads

Conclusion

The present report emphasizes the view that the acceleration of glycolysis occurring in islets stimulated by a rise in the extracellular concentration of glucose involves increases in both the availability of glycolytic intermediates, such as glucose-6-P and fructose-6-P, and the activity of the key glycolytic enzyme phosphofructokinase. We initially thought that such a dual mechanism could help to reconcile the substrate-site and regulatory-site hypotheses for the process of glucose-induced insulin release [33]. However, our more recent study on the properties of fructose-6-P,2-kinase now suggests that the increase in the rate of fructose-2,6-P2 synthesis may be mainly due to an elevation in the fructose-6-P content of the islet cells, an elevation itself attributable to a mass action phenomenon. We were also unable to detect any direct effect of glucose on the activity of phosphoglucomutase, the enzyme catalyzing the synthesis of glucose-1,6P2, another activator of phosphofructokinase. Therefore, there is as yet no convincing evidence that the molecule of glucose, whether located in the extracellular or intracellular fluid, interacts with a specific receptor to induce activation of a hypothetical glucosensor system. Obviously, the latter statement is not meant to deny that glucose binds to the stereospecific carrier mediating glucose transport across the B cell plasma membrane and to those enzymes (hexokinase, glucokinase, aldose reductase) catalyzing the first steps of its intracellular metabolism. Such binding phenomena, however, cannot be equated with the situation in which the insulin secretory response depends solely on the allosteric activation of an enzyme by a given secretagogue, as appears to be the case in islets stimulated by the nonmetabolized analogue of L-leucine, 2-aminobicyclo [2,2,12] heptane-2-carboxylic acid [34,35].

Keywords

Hexokinase Aldose Reductase Aldose Cell Plasma Membrane Phosphoglucomutase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • W. J. Malaisse
    • 1
  • F. Malaisse-Lagae
    • 1
  • A. Sener
    • 1
  1. 1.Laboratory of Experimental MedicineBrussels University Medical SchoolBrusselsBelgium

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