Journal of Plant Research

, Volume 113, Issue 2, pp 139–148

Histological Study of Seed Coat Development in Arabidopsis thaliana

  • Tom Beeckman
  • Riet De Rycke
  • Ronald Viane
  • Dirk Inzé

DOI: 10.1007/PL00013924

Cite this article as:
Beeckman, T., De Rycke, R., Viane, R. et al. J Plant Res (2000) 113: 139. doi:10.1007/PL00013924

Arabidopsis

seed coat development using light and transmission electron microscopy revealed major morphological changes associated with the transition of the integuments into the mature seed coat. By the use of a metachromatic staining procedure, cytological events such as the production of phenolic compounds and acidic polysaccharides were followed. Immediately after fertilization, the cells of the inner epidermis of the inner integument became vacuolated and subsequently accumulated pigment within them. This pigment started to disappear from the cytoplasm at the torpedo stage of the embryo, as it became green. During the torpedo stage, mucilage began to accumulate in the cells of the external epidermis of the outer integument. Furthermore, starch grains accumulated against the central part of the inner periclinal wall of these cells, resulting in the formation of small pyramidal domes that persisted until seed maturity. At the maturation stage, when the embryo became dormant and colourless, a new pigment accumulation was observed in an amorphous layer derived from remnants of crushed integument layers. This second pigment layer was responsible for the brown seed colour. These results show that seed coat formation may proceed in a coordinated way with the developmental phases of embryogenesis.

Keywords: Arabidopsis thaliana, Embryogenesis, Pigmentation, Seed coat 

Copyright information

© The Botanical Society of Japan 2000

Authors and Affiliations

  • Tom Beeckman
    • 1
  • Riet De Rycke
    • 1
  • Ronald Viane
    • 2
  • Dirk Inzé
    • 1
  1. 1.Department of Plant Genetics, Flanders Interuniversity Institute for Biotechnology, Ghent University, K.L. Ledeganckstraat 35, B–9000 Gent, BelgiumBE
  2. 2.Department of Biology, Ghent University, B–9000 Gent, BelgiumBE

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