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Journal of General Plant Pathology

, Volume 66, Issue 3, pp 264–268 | Cite as

Biological Control of Soft Rot of Chinese Cabbage Using Single and Mixed Treatments of Bacteriocin-producing Avirulent Mutants of Erwinia carotovora subsp. carotovora

  • Ampaabeng Gyedu KYEREMEH
  • Toshio KIKUMOTO
  • Duen-yau CHUANG
  • Yuichi GUNJI
  • Yoshiyuki TAKAHARA
  • Yoshio EHARA

Two pathogenic strains of E. carotovora subsp. carotovora 2T-2 and TT-4 with high bacteriocin activity but low sensitivity to the bacteriocins of other strains were treated with ethyl methane sulphonate (EMS). Two avirulent mutants A-f-39 and B-e-19 developed from 2T-2 and TT-4, respectively, by this treatment had the same bacteriocin activity as their respective parents and inhibited the in vitro growth of pathogenic strains of this species. The disease control of these two mutant strains were compared in the field in 1995 and 1997 to the control by CGE234M403 (M403) (a commercialized biocontrol agent), a mixture of A-f-39 and M403, and an agrochemical (basic dithianon-copper chloride). The protection obtained with A-f-39 was comparable to M403 and was better than that with the chemical. The mixture of A-f-39 and M403 consistently gave the best results in all the field trials.

Key words : soft rot disease, Chinese cabbage, bacteriocin-producing avirulent mutant, biological control. 

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Copyright information

© The Phytopathological Society of Japan 2000

Authors and Affiliations

  • Ampaabeng Gyedu KYEREMEH
    • 1
  • Toshio KIKUMOTO
    • 1
  • Duen-yau CHUANG
    • 1
  • Yuichi GUNJI
    • 1
  • Yoshiyuki TAKAHARA
    • 2
  • Yoshio EHARA
    • 3
  1. 1.Institute of Genetic Ecology, Tohoku Univesity, 2–1–1, Katahira, Aoba-ku, Sendai 980–8577, JapanJP
  2. 2.Central Glass Co. Ltd., Chemical Research Center, 2805 Imafuku-nakadai, Kawagoe 350–1151, JapanJP
  3. 3.Graduate School of Applied Life Science, Tohoku University, 1–1, Tsutsumidori Amamiyamachi, Aoba-ku, Sendai 981–8555, JapanJP

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