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Journal of General Plant Pathology

, Volume 66, Issue 3, pp 234–237 | Cite as

New Selective Medium for Isolation of Burkholderia glumae from Rice Seeds

  • Mitsuo KAWARADANI
  • Kiyotsugu OKADA
  • Shin'ichi KUSAKARI

A new selective medium for Burkholderia glumae was developed, which has a simpler composition and greater selectivity compared to Tsushima's S-PG medium currently used. This selective medium, designated CCNT, contains 2 g of yeast extract, 1 g of polypepton, 4 g of inositol, 10 mg of cetrimide, 10 mg of chloramphenicol, 1 mg of novobiocin, 100 mg of chlorotharonil and 18 g of agar in 1000 ml of distilled water, and is adjusted to pH 4.8. B. glumae produced a yellowish white colony with a diffusible yellow pigment on CCNT medium, which was distinguishable from other bacterial species when incubated at 41°C for 2 to 4 days. On CCNT medium, B. glumae was detected at a rate similar to that on S-PG medium in rice seeds, while other microorganisms were detected at a much lower rate.

Key words : Burkholderia glumae, selective medium, rice seed. 

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Copyright information

© The Phytopathological Society of Japan 2000

Authors and Affiliations

  • Mitsuo KAWARADANI
    • 1
  • Kiyotsugu OKADA
    • 1
  • Shin'ichi KUSAKARI
    • 1
  1. 1.Osaka Prefectural Agricultural and Forestry Research Center, Shakudo, Habikino 583–0862, JapanJP

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