Journal of General Plant Pathology

, Volume 66, Issue 3, pp 191–201 | Cite as

Molecular Phylogenetic Analysis of Ribosomal DNA Internal Transcribed Spacer Regions and Comparison of Fertility in Phomopsis Isolates from Fruit Trees

  • Satoko KANEMATSU
  • Nobuhiro MINAKA
  • Takao KOBAYASHI
  • Akira KUDO
  • Yoshihiro OHTSU

Sequences of the ribosomal DNA (rDNA) internal transcribed spacer (ITS) regions were examined to infer a molecular phylogeny of small-spored Phomopsis isolates, designated W-type (mainly white colony, weakly virulent, bearing both alpha and beta conidia at 25°C on PDA) and G-type (mainly gray colony, highly virulent, bearing only alpha conidia at 25°C on PDA), and P. amygdali from fruit trees. Phomopsis G-type and P. amygdali were a monophyletic group distinct from the W-type. The W-type isolates were divided into two monophyletic groups. Diaporthe citri, D. tanakae, P. asparagi, P. viticola, P. vitimegaspora and D. nomurai, which are morphologically distinguishable from W- and G-types, differed from the W- and G-types in molecular phylogenetic analyses. PCR-RFLP analysis of rDNA ITS regions was useful to distinguish each of the Phomopsis species and groups using three restriction enzymes. In mating tests, W-type isolates from fruit trees were heterothallic and inter-fertile even between isolates belonging to the different monophyletic groups. Isolates of the G-type and P. amygdali collected in Japan were cross-fertile. Some isolates from Lunaria annua, Ulmus glabra and Juglans regia belonged to one of the two monophyletic groups of the W-type and were cross-fertile with W-type isolates from Rosaceous fruit trees.

Key words : Diaporthe eres, Diaporthe medusaea, Phomopsis amygdali, rDNA ITS regions, mating, taxonomy. 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© The Phytopathological Society of Japan 2000

Authors and Affiliations

  • Satoko KANEMATSU
    • 1
  • Nobuhiro MINAKA
    • 2
  • Takao KOBAYASHI
    • 3
  • Akira KUDO
    • 1
  • Yoshihiro OHTSU
    • 1
  1. 1.National Institute of Fruit Tree Science, Tsukuba 305–8605, JapanJP
  2. 2.National Institute of Agro-Environmental Sciences, Tsukuba 305–8604, JapanJP
  3. 3.Department of International Agricultural Development, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156–8502, JapanJP

Personalised recommendations