Cellular and Molecular Life Sciences CMLS

, Volume 59, Issue 11, pp 1972–1982 | Cite as

Differential influences of various arsenic compounds on glutathione redox status and antioxidative enzymes in porcine endothelial cells

  • J. Y. Yeh
  • L. C. Cheng
  • B. R. Ou
  • P. D. Whanger
  • L. W. Chang

Abstract.

The cellular response and detoxification mechanisms in porcine endothelial cells (PAECs) to arsenic trioxide (As2O3), sodium arsenite (NaAsO2) and sodium arsenate (Na2HAsO4) were investigated. NaAsO2 at 20 μM for 72 h increased Cu/Zn superoxide dismutase activity resulting in elevated intracellular hydrogen peroxide levels, but As2O3 and Na2HAsO4 did not. Trivalent arsenic compounds increased intracellular oxidized glutathione (GSSG) and total glutathione (GSH) and cellular glutathione peroxidase (cGPX) and glutathione S-transferase (GST) activity, but not glutathione reductase activity. The increased cGPX activity resulted in an elevated cellular GSSG content. Na2HAsO4 increased the cellular GSSG level at 72 h compared to controls. These results imply that the increased GSH content responding to the oxidative stress by trivalent arsenic compounds may be mainly related to the regulation of GSH turnover. The increased GST activity implies that the elevated intracellular GSH level responding to the oxidative stress may be used to conjugate arsenic in PAECs and facilitate arsenic efflux.

Key words. Arsenic; antioxidative enzyme; glutathione redox status; hydrogen peroxide; porcine endothelial cell. 

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Copyright information

© Birkhäuser Verlag, 2002

Authors and Affiliations

  • J. Y. Yeh
    • 1
  • L. C. Cheng
    • 1
  • B. R. Ou
    • 2
  • P. D. Whanger
    • 3
  • L. W. Chang
    • 1
  1. 1.Division of Environmental Health and Occupational Medicine, National Health Research Institutes, Room 108, Medical Research Building, 100 Shih-Chuan 1st Road, Kaohsiung, 807, Taiwan (R.O.C.), Fax + 886 7 3221912, e-mail: jyyeh@nhri.org.twTW
  2. 2.Department of Animal Science, Tunghai University, Taichung, Taiwan (R.O.C.)TW
  3. 3.Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon (USA)US

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